期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
比阿培南等抗生素诱导铜绿假单胞菌释放内毒素的实验性研究
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1. 河北中医学院 药学院, 河北 石家庄 050200;
2. 河北中医学院 实验中心, 河北 石家庄 050200;
3. 河北医科大学第四医院 药剂科, 河北 石家庄 050011;
4. 河北中医学院 护理学院, 河北 石家庄 050200 -
DOI：10.11826/j.issn.1000-5854.2014.04.016
Experimental Study on the Release of Endotoxin from Pseudomonas aeruginosa Induced by Antibiotics Biapenem,Meropenem,Levofloxacin,and Ceftazidime
摘要/Abstract
研究比阿培南等抗生素诱导铜绿假单胞菌（Pseudomonas aeruginosa，Pa.）释放内毒素（lipopolysaccharide，LPS）的作用机制.比阿培南（biapenem，BIPM）、美罗培南（meropenem，MEM）、左氧氟沙星（levofloxacin，LVFX）、头孢他啶（ceftazidime，CAZ）4种药物以不同质量浓度作用于Pa.，采用鲎试剂动态浊度法检测不同时间点的内毒素含量；检测各药物在0.5MIC作用下的细菌数量变化，并观察作用7h后细菌的形态学改变.与对照组比较，各给药组释放的LPS均明显降低.4种抗生素作用于Pa.，导致细菌形态和数量发生改变，各给药组的细菌数量均低于对照组.细菌形态学研究显示，CAZ和LVFX组细菌形态呈丝状，BIPM组呈椭圆形，而MEM组可观察到球形和丝状菌共存.LPS释放水平与药物种类、药物浓度、作用时间等因素相关，4种药物导致细菌形态改变各异，提示其诱导铜绿假单胞菌释放LPS的作用机制不同.
To study the mechanisms of endotoxin lipopolysaccharide (LPS) release from Pseudomonas aeruginosa(Pa.)induced by biapenem (BIPM),meropenem (MEM),levofloxacin (LVFX),and ceftazidime (CAZ). Pa. was exposed to different concentrations of BIPM,MEM,LVFX,or CAZ for different time,and the LPS release of Pa. was measured by tachypleus amebocyte lysate test (TAL test).Quantities of bacteria exposed to 0.5MIC antibiotics were measured.The morphology of bacteria treated with different antibiotics for 7 hours was evaluated by light microscope.LPS releases of Pa. treated with the four kinds of antibiotics were significantly less than control group.Compared with control group,exposion to the four antibiotics caused changes in morphology and decrease in cell quantities of Pa..The Pa. cells treated with CAZ or LVFX became long filamentous shape.The bacteria exposed to BIPM appeared elliptical.At the same time,spherical cells and long filamentous bacteria coexisted for MEM-treated Pa..The level of LPS releases is influenced by the kind of antibiotics,their concentrations and incubation time.All of the four antibiotics could induce morphological changes of Pa.,but to various shapes.It may be related to the different mechanisms involved in the LPS release induced by different antibiotics.
关键词
参考文献 16
- [1] 陈宏斌,赵春江,王辉,等.2011年中国13家教学医院院内感染常见病原菌耐药性分析[J].中华内科杂志,2013,52(3):203-212.
- [2] 黄赛虎,华军,谢敏慧,等.儿科重症监护病房脓毒血症临床及病原学分析[J].儿科药学杂志,2012,18(10):31-33.
- [3] 张海英,熊盛道,熊维宁.抗菌药物单一或联合用药诱导铜绿假单胞菌释放内毒素的比较性研究[J].中华微生物学和免疫学杂志,2006,26(1):18-22.
- [4] 王红芳,许力军,牛晓莉,等.比阿培南等抗生素诱导鲍曼不动杆菌释放内毒素的实验研究[J].天津医药,2013,41(10):957-961.
- [5] 李耘,吕媛.琼脂二倍稀释法测定抗菌药物的最低抑菌浓度[M]//魏伟,吴希美,李元建.药理学实验方法学.4版.北京:人民卫生出版社,2010:1436-1439.
- [6] 国家药典委员会.2010版中华人民共和国药典[S].北京:中国医药科技出版社,2010(附录):99-102.
- [7] TSUJI M,MATSUDA H,MIWA H.Antimicrobial Induced Release of Endotoxin from Pseudomonas aeruginosa:Comparison of in Vitro and Animal Models[J].J Antimicrob Chemother,2003,51(2):353-359.
- [8] HORII T,ICHIYAMA S,OHTA M,et al.Relationship Between Morphological Changes and Endotoxin Release Induced by Carbapenems in Pseudomonas aeruginosa[J].J Med Microbiol,1999,48(3):309-315.
- [9] HORII T,MURAMATSU H,MONJI A,et al.Release of Exotoxin A,Peptidoglycan and Endotoxin After Exposure of Clinical Pseudomonas aeruginosa Isolates to Carbapenems in Vitro[J].Chemotherapy,2005,51(6):324-331.
- [10] 易林高,李佳,林洁,等.头孢菌素类抗生素的特点及临床应用[J].海峡药学,2010,22(3):159-161.
- [11] 张凤凯,金少鸿.β-内酰胺类抗生素的作用靶位——青霉素结合蛋白[J].国外医药:抗生素分册,2000,21(3):111-115.
- [12] 王顺宾,黄逢元,吴伯瑜.不同抗生素低浓度下引发细菌释放内毒素的研究[J].中华医学杂志,1999,79(5):396-397.
- [13] TOSHINOBU Horii,MIYA Kobayashi,MASAYUKI Nadai,et al.Carbapenem-induced Endotoxin Release in Gram-negative Bacterial Sepsis Rat Models[J].FEMS Immunology & Medical Microbiology,1998,21(4):297-302.
- [14] 陈雪梅,周向东.不同抗菌药物杀菌过程导致细菌内毒素释放的异质性[J].中华医院感染学杂志,1999,9(1):19-21.
- [15] BUIJS J,DOFFERHOFF A S M,MOUTON J W,et al.Concentration-dependency of β-lactam-induced Filament formation in Gram-negative Bacteria[J].Clin Microbiol Infect,2008,14(4):344-349.
- [16] 周向东,阳涛.抗菌药物杀菌过程致内毒素释放的比较性研究[J].中华内科杂志,1998,37(10):686-688.