An analytical method to simultaneously determinate nine kinds of sulfonamide residues in water environment by SPE-HPLC was established.We selected the Oasis HLB solid phase extraction column to research the effects of different pH values,elution solvents,and elution volume on extraction abilities.The mobile phase was composed of acetronitrile and 0.1 % formic acid(gradient elution) with a detection wavelength of 268 nm.The results indicated that the instrument detection limit of nine kinds of sulfonamides from 64.9 to 300 μg/L,which revealed the stability and accuracy of the instrument.The recoveries of sulfonamides from pure water,tap water,sewage and sewage effluents were 13.6 %~93.7 %,22.4 %~87.4 %,n.d.~60.1 % and n.d.~63.9 %(n.d.=not detected) respectively.All the recoveries were greater than 50 % except sulphaguanidine.