期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
多重PCR检测食源金黄色葡萄球菌毒素基因的建立及应用
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1. 河北国际旅行卫生保健中心;
2. 石家庄市桥东区疾病预防控制中心;
3. 石家庄市疾病预防控制中心 -
DOI：
Multiplex PCR for Detection of Toxin Genes in Food-borne Staphylococcus aureus and Its Application
摘要/Abstract
建立了快速检测食源性金黄色葡萄球菌(SA)16SrDNA、纤维蛋白结合蛋白(clfa A和clfa B)、纤连蛋白结合蛋白(fnbp A和fnbp B)的多重PCR方法.利用GenBank SA的16SrDNA,clfa A,clfa B,fnbp A和fnbp B基因序列,设计5对特异性引物,建立鉴定SA及分析SA毒素基因的五重PCR方法,并对160株食源SA进行属鉴定和毒素基因检测.结果显示,供试的160株食源SA中葡萄球菌属16SrDNA鉴定均为阳性;4种SA毒素基因检出率由高到低依次为clfa A(91.88%),fnbp A(21.88%),clfa B(19.38%),fnbp B(8.13%).该方法简便、快捷、准确,为食源SA的属鉴定和毒素基因分析提供了快速检测方法,同时可作为食源性疾病事件处理的溯源技术.
According to gene sequences of 16SrDNA,clfa A,clfa B,fnbp A and fnbp B of Staphylococ-cusa ureus（SA）in GenBank,five pairs of specific primers were designed and used in multiplex PCR for i-dentification and detection of SA toxin genes.Multiplex PCR was developed and optimized in the detection of 160food-borne SA isolates.The positive rates for detection of 16SrDNA,clfa A,clfa B,fnbp A and fnbp B genes by PCR were 100%,91.88%,19.38%,21.88%and 8.13%,respectively.The results indicated that multiplex PCR assay was convenient,time-saving and accurate.Our study has provided a rapid detec- tion method for identification of food-borne SA,molecular analysis of toxins in SA,and the investigation of the origin of food-borne diseases.