期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Fluorescence Study on the Interaction Between 5-sulfosalicylic Acid and Bovine Serum Albumin
摘要/Abstract
用荧光法研究了5磺基水杨酸(SSA)与牛血清白蛋白(BSA)之间的相互作用.实验发现SSA可以猝灭BSA的荧光,同时自身的荧光敏化增强,表明两者之间发生了能量转移.能量转移的机理是BSA与SSA结合形成了复合物.SSA在BSA212位色氨酸残基附近的结合数为1,结合常数为6.5×105.疏水力是结合反应的主要作用力.基于Forster能量转移理论确定了SSA与BSA212位色氨酸残基间的距离为1.55nm.研究了实验条件对反应的影响,在pH4.0～8.0之间,BSASSA复合物基本稳定,离子强度对复合物形成有明显影响.
Interaction between 5-sulfosalicylic acid (SSA) and bovine serum albumin (BSA) was studied by fluorescence method.It was found that fluorescence of BSA were quenched by SSA,meanwhile the fluorescence of SSA enhanced,indicating that an energy transfer between BSA and SSA had been occurred.The mechanism of energy transfer was considered to be the formation of BSA-SSA complex.The binding number of SSA on the vicinity of 212-tryptophane residue of BSA was determined to be 1,and the binding constant was 6.5×10~5.Hydrophobic force is the main binding force between SSA and BSA.Based on Forster energy transfer theory,the distance between 212-tryptophane residue of BSA and SSA was determined to be 1.55*!nm.Effects of experimental conditions on the interaction were investigated.At pH 4.0～8.0,BSA-SSA complex were essentially stable,ionic strength had a significant influence on the formation of complex.
关键词
参考文献 11
- [1] GRINNELL J.The Niche Relationship of the California Thrasher [J].Auk,1917,34:27-43.
- [2] ELTON C.Animal Ecology [M].London:Sidgwick and Jackson,1927:63-68.
- [3] ODUM E P.Basic Ecology [M].New York:CBS College Publishing,1983.
- [4] HUTCHINSON G E.Concluding Remarks [J].Cold Spring Harbor Symposia on Quantitative Biology,1957,22:415-427.
- [5] WHITTAKER R H,LEVIN S A,ROOT R B.Niche [J].Habitat and Ecotype American Naturalist,1973,7(5):32-33.
- [6] LEIBOLD M A.The Niche Concept Revisited:Mechanistic Models and Community Context [J].Ecology,1995,76(5):1371-1382.
- [7] 王刚,赵松岭,张鹏云,等.关于生态位定义的探讨及生态位重叠计测公式改进的研究 [J].生态学报,1984,4(2):119-126.
- [8] 马世骏.现代生态学透视 [M].北京:科学出版社,1990:72-89.
- [9] 李向明.略论旅游资源生态位的概念与选择策略 [J].旅游学刊,2007(2):50-53.
- [10] 朱春全.生态位势态理论与扩展假说 [J].生态学报,1997,17(3):324-332.
- [11] 金松岩,张敏,杨春.生态学理论研究 [J].内蒙古环境科学,2009,21(4):12-15.