期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
微小隐孢子虫表面抗原CP23 DNA疫苗免疫山羊诱导免疫应答及保护性研究
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1. 解放军军需大学动物科技系, 吉林 长春 130062;
2. 第一军医大学实验动物中心, 广东 广州 510515;
3. 河北经贸大学生物工程系, 河北 石家庄 050061 -
DOI：
The Protective Immune Responses Induced by CP 23 DNA Vaccine of Cryptosporidium parvum in Goats
摘要/Abstract
观察微小隐孢子虫 (Cryptosporidium parvum)子孢子表面抗原 CP2 3 DNA疫苗免疫山羊诱导其产生免疫应答和保护性作用 .将重组质粒 p CR 3 .1 2 3经鼻粘膜免疫怀孕山羊, 用 ELISA测定 Ig G和 Ig A抗体滴度, 用 C.parvum卵囊经口对其后代攻虫感染 .抗 CP 2 3抗体存在于免疫山羊的血浆和初乳中, 且抗体滴度随免疫时间延长而增高 .C.parvum卵囊经口感染后代, 试验组山羊后代与对照组相比, 卵囊排出数量减少, 排出时间缩短 .微小隐孢子虫表面蛋白 CP2 3 DNA疫苗能诱导山羊产生特异性免疫应答并对其后代有一定免疫保护作用
To observe the protective immune respo nses induced by the CP 23 DN Avaccine of C.parv um in g oat s. Largescale preparation of the recombina nt plasmids pCR 3. 1-23 was ino culated bynasal mucosa of conceived goats. The Ig G and Ig A antibodies in serum and colostrum were detected byenzyme linked immunosorbent assay （ ELISA ） . The lambs of vaccinated goats were challenged byo ocy st s of C. parvum. The speci fic Ig G and IgA a ntibodies in serum a nd co lostrum f rom vaccinatedg oated could be detected and the ti ter of IgG and Ig A g radually rose wi th time pro long . The o ocy stsnumber and shedding time of the lambs from vaccinated goats were sho rter than of cont rol g roups afterlambs w ere challeng ed wi th oocyst s of C. parvum. The C P23 DN A v accine of C. parvum could elicitimmune responses against C. parv um oocysts.