期刊信息

  • 刊名: 河北师范大学学报(自然科学版)Journal of Hebei Normal University (Natural Science)
  • 主办: 河北师范大学
  • ISSN: 1000-5854
  • CN: 13-1061/N
  • 中国科技核心期刊
  • 中国期刊方阵入选期刊
  • 中国高校优秀科技期刊
  • 华北优秀期刊
  • 河北省优秀科技期刊

南方红豆杉细胞培养中紫杉醇代谢调节的研究

  • 河北师范大学生命科学学院 河北 石家庄050016
  • DOI:

Study on Suspension Culture and Its Taxol Production of Taxus mairei

摘要/Abstract

摘要:

对南方红豆杉悬浮培养细胞的生长动力学和紫杉烷类物质积累的动力学进行了探讨.紫杉醇合成途径中由巴卡亭Ⅲ生成紫杉醇的反应是受阻的.加入pH稳定剂MES可以减少巴卡亭Ⅲ向胞外的分泌,提高了紫杉醇总量和在细胞内的积累.

Abstract:

The relationship of the growth of cells and the production of taxol was showed negative-related through the research. To conclude the combine of the main and side chain was blockd. The secretion of bacctin Ⅲ was decrease and the production of total taxol and taxol in cells was improved when MES was added to medium.

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