期刊信息
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- 刊名: 河北师范大学学报(自然科学版)Journal of Hebei Normal University (Natural Science)
- 主办: 河北师范大学
- ISSN: 1000-5854
- CN: 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Cloning and Expression of Alkaline Lipase Gene from Peniciuium cyclopium Mutant
摘要/Abstract
摘要:
圆弧青霉(Peniciuiumcyclopium)突变株可产生一种具有工业价值的碱性脂肪酶(alkalinelipase,PEL),通过RT PCR基因克隆及序列测定,获得了PEL完整的基因序列.该基因全长855bp,编码1个由285个氨基酸残基组成的酶蛋白.根据氨基酸组成推导该脂肪酶蛋白的分子量约为30kD.
Abstract:
Peniciuium cyclopium mutant could produce an alkaline lipase (PEL) with industrially value.A 1050bp PEL gene was amplified by RT-PCR and other methods of molecular biology,and analysis of the nucleotide sequence indicated that the PEL gene contained a complete open reading frame encoding a protein of 285 amino acid residues.The expressed PEL molecular weight of mature peptide is 30kD
关键词
Key words:
Peniciuium cyclopium
;
alkaline lipase
;
gene cloning
;
gene expression
;