In this experiment, Oryza sativa ssp. japonica Nippobare mature seeds were used to initiate calli and NB media supplemented with different concentrations and combinations of 2, 4-D(2, 4-dichlorophenoxy acetic acid) and 6 BA(6 benzylaminopurine)was used for callus initiation. The effects of the two plant horome were analyzed by counting the initiation rate and regeneration rate. The results showed that 2,4-D was a necessary factor in callus initiation,the media with both 2,4-D and 6 BA would have a better effect on mature embryo culture. For Oryza sativa ssp. japonica Nippobare 2. 0 mg/ L of 2,4-D and 0. 5 mg/ L of 6 BA added in culture media express the highest initiation rate ;2. 0 mg/ L of 2,4-D and 0. 2 mg/ L of 6 BA added in culture media express the highest regeneration rate and culture ability.