期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Construction and Detection of a cDNA Library of Wheat Seedling Salt Stressed for 72 Hours
摘要/Abstract
摘要：
以98-160盐胁迫72 h叶片的mRNA为起始材料,采用SMART技术构建了一个高质量的cDNA文库,经检测,未扩增文库的滴度为3.44×106pfu/mL,白斑率>85%,插入片段多数在0.5~1.5 kb,有的可以达到2 kb,平均长度为800 bp.因此,按照此方法构建的是1个高质量的cDNA文库,可有效地用于下一步全长基因的筛选和克隆.
Abstract：
A SMART cDNA library was constructed by using the leaves of 98-160 stressed for 72 hours.Quality detection results showed that the titer of the unamplified cDNA library was 3.44×106 pfu/mL(white plague ratio>85%),the insert varied from 0.5 to 1.5 kb with the average of 800 base pairs,some were even larger than 2 kb.Therefore the cDNA library constructed according to the above method was a high-quality cDNA library which could be used for further full-length gene screening and cloning efficiently.
关键词
Key words：
wheat
;
seedling
;
salt stress
;
cDNA library