期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
FSK88对UMR106细胞中cbfa1基因表达的影响
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1. 中国科学院 动物研究所 生物膜与膜工程国家重点实验室, 北京 100080;
2. 中国科学院 研究生院, 北京 100039 -
DOI：
Effect of FSK88 on cbfa1 Gene Expression in Rat Osteosarcoma UMR106 Cell
摘要/Abstract
以大鼠成骨肉瘤细胞———UMR106为模型,应用RT-PCR方法,检测用不同浓度的FSK88药液(25,50,100μmol/L)、维甲酸(RA,10μmol/L,阳性对照)以及FSK88+RA(等体积)混合药液处理细胞72 h后,细胞内cbfa1基因表达量的变化.3次重复实验显示:与对照组相比,经25,50,100μmol/L 3种浓度FSK88药液处理的细胞内cbfa1基因表达量分别增加35.5%,64.5%,124.0%.其中用100μmol/L的FSK88处理细胞后,cbfa1基因表达量有显著的上调(P<0.01),并且上调作用强于10μmol/L的维甲酸药液.证明FSK88能够通过直接或间接增强UMR106细胞中cbfa1基因的表达,从而促进肿瘤细胞向正常细胞逆转分化.
Expression of cbfa1 gene in rat osteosarcoma UMR106 cell was detected by RT-PCR after the 72 h's treatment of FSK88 liquid with different mol concentration(25,50,100 μmol/L),Retinoic Acid(RA,10 μmol/L)and mixed liquid of FSK88 and RA(1∶1).The results of three repeated experiments demonstrated that:compared with the control group,cbfa1 gene expression of the cells treated with 25,50,100 μmol/L FSK88 had an increase of 35.5%,64.5%,124.0% respectively.Especially after treatment of 100 μmol/L FSK88 liquid,UMR106 cell showed an significantly enhancive cbfa1 gene expression(P<0.01),and the increase rate was bigger than the cells treated with 10 μmol/L Retinoic Acid.It was provedthat FSK88 could induce cancer cells to differentiation through enhancing the expression of cbfa1 gene directly or indirectly.