期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
新生大鼠大脑皮层和海马神经元体外培养方法及改进
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1. 河北师范大学分子神经生物学与神经药理学研究所, 河北石家庄 050016;
2. 河北化工医药职业技术学院化工系, 河北石家庄 050031 -
DOI：
The Method and Improvement of Primarily Culturing Neurons from the Cerebral Cortex and Hippocampus of Newborn Rats
摘要/Abstract
探索用24 h内新生SD大鼠进行神经元体外培养的方法。取新生SD大鼠的大脑皮层和海马，胰酶消化5~10 min后，放入含100 g/L血清的DMEM/F12培养基中培养。24 h后，更换含B 27无血清培养基。以后每隔1 d，半换液。结果显示绝大部分神经元于第2天长出突起，第3天神经元间形成复杂的联系，第7天神经元发育基本成熟。经神经元特异性烯醇酶(NSE)鉴定，培养细胞均为神经元，纯度可达90%以上，可以用于实验。
In order to get the method of primary neuronal culture of newborn rats.The cerebral cortex and hippocampus were dissected from the newborn rat,dissociated by trypsinization,and plated onto coverslips in DMEM/F12,supplemented with 100 g/L serum.Twenty-four hours after plating,the medium was replaced with medium containing B 27 without serum,and replaced half medium every two days.The most neurons get dendrites and axon on the second day.On the third day,the touch formed among neurons.Neurons matured in the seventh day.After identified by neuron-specific enolase,the purity of the cultural neurons was more than 90 %.Then cells were used to experiment.