期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Molecular Cloning and Characterization of Hepcidin Mature Peptide from Soft-shelled Turtle and Preliminary Study on Its Homology
摘要/Abstract
采用同源克隆的方法,以用于扩增大菱鲆hepcidin成熟肽基因的引物,从中华鳖肝脏cDNA中扩增得到1条长78 bp的基因片段.初步判断其属于Hepcidin家族,与HAMP1具有较高的同源性,分析表明其为Hepcidin成熟肽.根据基因序列推断得到的成熟肽蛋白序列如下:QSHISLCRWCCNCCKANKGCGFCCKF.与其他物种的成熟肽进行比对,发现其与大菱鲆的成熟肽序列同源性达到100%,并构建了基于各物种Hepcidin前体肽的系统发育树,为研究物种进化提供了证据.
A fragment of 78 bp is amplified from the soft-shelled turtle liver cDNA using homology-based cloning methods with primers used for amplification of turbot hepcidin mature peptide gene.Initial evaluation reveals that it belongs to hepcidin family, and having a high homology with HAMP1.Analysis indicates that it may be hepcidin mature peptide.The amino acid sequence of mature peptide deduced from the gene f ragment sequence is QSHIS LCRWCCNCCKANKGCGFCCKF.After alignment by blast, the sequence shares 100% homology with turbot.A phy logenetic tree,which may provide evidence fo r species evolution reaserch, is constructed based on the hepcidin propeptide alignment.
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参考文献 11
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