期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
The Characteristic Analysis of Human Adipose- derived Stem Cells Transfected with Oct-4 Gene
摘要/Abstract
观察了转染Oct-4基因对人脂肪来源干细胞性状的影响.首先将Oct-4基因全序列亚克隆至逆转录病毒载体pMSCVneo中,构建逆转录病毒重组质粒,然后以脂质体介导转染包装细胞PT67,成功建立PT67-Oct4细胞系,随后转染人脂肪来源干细胞(adipose-derived stemcells,ADSCs)中,最后对高表达Oct-4基因的ADSCs性状进行观察.实验结果表明,对高表达Oct-4蛋白的ADSCs-Oct4同未转染Oct-4的ADSCs相比,ADSCs-Oct4在形态学、表面标志、核型等方面无明显差别,接种ADSCs-Oct4细胞8周后裸鼠体内未形成肿瘤.以上结果说明ADSCs-Oct4细胞遗传性状稳定,无致瘤性,为深入开展ADSCs研究提供了实验依据.
The effects of Oct-4 gene transfection on human adipose-derived stem cells (ADSCs) were observed.At first,the whole sequence of Oct-4 gene was sub-cloned into retroviral vector pMSCVneo to construct the recombined ret rovirus vector pMSCV-Oct4,and then the recombined plasmid was transferred into packaging cell line PT67 with lipofectamine.Finally PT-Oct4 cell lines were successfully established. ADSCs were transfected and observed.The results showed that the ADSCs-Oct4 had no obvious dif ference in morphology,surface marker and karyotype compared to unt ransfected ADSCs.Furthermore,there was no tumor formed in nude mice 8 weeks after ADSCs-Oct4 inoculation. The above results revealed that the ADSCs-Oct4 had stable genetict raits,no tumorigenicity,and provided the experimental basis for in-depth research.