期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Cloning and Expression of Endo-beta-1, 4-glucanase Gene of the Head of Termites
摘要/Abstract
对中国广泛分布的黑翅土白蚁(Odontotermes formosanus)头部内切β-1,4葡聚糖酶(endo-beta-1,4-glucanase)基因进行克隆,并在原核生物大肠杆菌体内进行了表达.根据Genbank上已报道的endo-beta-1,4-glu-canase基因设计引物,通过RT-PCR获得1个完整的内切β-1,4葡聚糖酶基因.通过基因测序以及基因比对发现与Genbank上发表的内切β-1,4葡聚糖酶同源性高达96.40%.将获得的基因导入大肠杆菌体内进行诱导表达,表达出的蛋白经Western-blot检测,为该基因所表达的目的蛋白.实验为进一步对内切β-1,4葡聚糖酶的实际应用奠定一定的实验基础.
Endo-beta-1,4-glucanase gene,which was expressed in the head of Odontotermes formosanus wildly distributed in China,cloned and expressed in E.coli bacteria.A pair of specific primers of the endobeta-1,4-glucanase gene,which was designed according to Genbank database,was used to amplify the complete endo-beta-1,4-glucanase gene by RT-PCR technology.Through gene sequencing and comparing with the endo-beta-1,4-glucanase gene publicated in the Genbank database,the homology of the endo-beta-1,4glucanase gene in the research achieved as high as 96.40%.The endo-beta-1,4-glucanase gene was inserted to the prokaryotic expression system,and induced to translate into protein in the suitable condition.The expression protein was analysed by Western-blot,the result indicated that the endo-beta-1,4-glucanase gene was translated into the objective protein.This research is an experimental foundation for the advanced application study of the endo-beta-1,4-glucanase.