The methods for the culture of transgenic E. colt and purification of plant:almodulin from the above engineering bacteria were investigated. It was shown that the3unable density of engineering bacteria as judged by A,,, is from 0. 5-0. 7,as the engineering bacteria was cultured in LB liquid medium containing 100 mg/L kanamycin and25 mg/L chloramphenicol. The calmodulin in the engineering bacteria was purified byphenyl-sepharose 4B affinity chromatography and the purity was judged by SDS PAGE,about 20 mg of purified calmodulin was able to be purified from 1 liter of the engineering bacteria.