The male sterile lines and their corresponding maintainers of wheat were treated under higher temperature. After that the mitochondrions polypeptides and the dissolved proteins were abstracted and analyzed by SDS-PAGE. It was found that the mitochondrions polypeptides of male sterile lines and corresponding maintainers all tended to the same SDS-PAGE patterns as each others. The unsteady A-type Fan91 male sterile line showed the significant fertility restoring after heating shock. But the steady CMS-T lines and their maintainers didn 't show the changing of fertility after short time 's heating shock. Analyzed the natural control 's SDS-PAGE patterns,it was found that the mitochondrions polypeptides of CMS-T, A-type male sterile lines decreased significantly contrast with their corresponding maintainers. That is, the structure and function of the mitochondrions in male sterile lines 'pollens had lost significantly when the pollens developed from uninuclear period to binuclear period.