期刊信息
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- 刊名: 河北师范大学学报(自然科学版)Journal of Hebei Normal University (Natural Science)
- 主办: 河北师范大学
- ISSN: 1000-5854
- CN: 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
薄层色谱法分离测定1, 5-苯并硫氮杂α-氨基-β-内酰胺
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1. 河北师范大学 化学系, 河北 石家庄 050016;
2. 河北南戴河师范学校, 河北 秦皇岛 066000 -
DOI:
Determination of 1, 5 -benzothiazepines-α -amino-β-lactam with Thin-layer Chromatography
摘要/Abstract
摘要:
用薄层色谱法分离测定了反应混合物中的1, 5-苯并硫氮杂草-α-氨基-β-内酰胺.扫描条件为:λ1=280 nm, λ2=354 nm, 狭缝1.2 mm× 1.2 mm, SX=3.校准曲线的线性范围是 0.275~7.70μg(γ=0.990 5, n=9), 最低检出限为0.140μg.方法的变异系数小于5%, 平均回收率为100.15%, 方法简便、快速.
Abstract:
A method for separation and determination of 1, 5-benzothiazepines T-amino U-lactam in reaction mixture by thin-layer chromatography has been established. The operating conditions are: λ1= 280nm, λ2= 354nm, beam size 1.2mm× 1.2mm, SX= 3. The calibration curves are linear in the range of 0.275 ~7.70μg(V = 0.9905, n= 9), the detectable limit is 0.140 μg. The coefficient of variation of this method is less than 5 %, the average recovery for U-lactam is 100. 15 %. The method is simple and rapid.
关键词
Key words:
thin-layer chromatography
;
U-lactam
;
benzothiazepion