期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
继代时间对南方红豆杉细胞生长和产生紫杉醇的影响
- 河北师范大学生命科学学院; 河北师范大学生命科学学院 河北石家庄050016; 河北石家庄050016; 河北石家庄050016;
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DOI：
Effects of Subculture Time on the Growth and Taxol Production in Taxus chinensis var. mairei Cel
摘要/Abstract
通过激素组合正交实验及继代时间的选择对南方红豆杉细胞增殖培养条件进行了优化研究.结果表明在细胞生长对数期继代比静止期继代细胞增长率提高1.6倍,而紫杉醇产量差异不大.同时在对75个激素处理的筛选实验中证实NAA与6-BA的组合优于2,4-D与6-BA的组合,其中NAA1mg/L+6-BA1mg/L的处理可获得最大增长率.
Optimizing proliferation conditions in Taxus chinensis var. mairei cell culture was stud-ied by experimental orthogonal design of phytohormone combinations and optimizing subculture time.The results indicated that the growth rate of cells subcultured at exponential stage was 1.6times of that at stationary stage and there was no significant difference in the taxol content.In addition,it was proved that combination of NAA and 6-BA was superior to that of 2,4-D and 6-BA and the greatest growth rate of cell was obtained with the combination of 1mg/ L NAA and 1mg/ L 6-BA among the choice of 75phytohormone treatments.