期刊信息
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- 刊名: 河北师范大学学报(自然科学版)Journal of Hebei Normal University (Natural Science)
- 主办: 河北师范大学
- ISSN: 1000-5854
- CN: 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
3月龄大鼠不同大动脉光镜和CLSM观察及定量分析
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1. 河北师范大学生命科学学院, 河北 石家庄050016;
2. 石家庄市第11中学, 河北 石家庄050000 -
DOI:
LM and CLSM Observation and Quantitative Analysis of Different Large Arteries in Rats of Three Months Old
摘要/Abstract
采取常规石蜡切片和HE染色法制成切片标本,在光镜和激光共聚焦扫描显微镜(CLSM)下测量升主动脉、胸主动脉、腹主动脉和股动脉段的管壁中膜厚度、管腔面积、管壁中膜面积、弹性膜层数和弹性膜荧光强度等.结果表明了由升主动脉至股动脉呈现血管壁中膜变薄、管腔缩小、弹性膜荧光强度降低等变化规律.
In this experiment,conventional hematoxylin-eosin staining was used to make sections,then with light microscope and confocal laser scanning microscope the changes of different elastic ar-teries were investigated and measured.The results are as follows:from ascending aoerta to femoral artery,both the area and the thickness of tunica media of vascular wall were obviously decreased,so was the area of vascular cavity;while the ratio of vascular wall area to vascular cativity area was obvi-ously increased;fluorescence intensity of elastic membranes showed a tendency to decrease.In a word,great changes take place in different elastic arteries.