期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Establishment of Tissue Culture and Rapid Proliferation System for Medical Plant Platycodon grandiflorum
摘要/Abstract
以药用植物(桔梗)种发无菌苗幼嫩茎尖、茎段为外植体,附加不同种类、不同质量浓度的激素进行组织培养,建立了快速繁殖体系.得出如下最佳培养基组合:诱导分化培养基MS+6-BA 1.0 mg/L+NAA 0.05 mg/L;增殖培养基MS+6-BA 0.4 mg/L+NAA 0.1 mg/L;生根培养基1/2 MS+NAA 0.5 mg/L和1/2 MS+IBA 0.5 mg/L.为桔梗优良株系的繁育和利用提供了有效途径.
To establish the rapid proliferation system of the medical plant Platycodon grandiflorum.The shoot apices and segment of P.grandiflorum were used as explants and cultured on different media supplemented with various portion of phytohormone.MS medium containing 6-BA 1.0 mg/L and NAA0.05 mg/L was the best for callus induction;MS medium containing 6-BA 0.4 mg/L and NAA 0.1 mg/Lwas suitable for crowd bud propagation;1/2 MS medium containing IBA 0.5 mg/L and 1/2 MS medium containing NAA 0.5 mg/L were suitable for inducing root.The rapid proliferation of P.grandiflorum by tissue culture method could be established,which will provide the effective ways of the breeding and clone of excellent varieties and utilization of P.grandiflorum.