期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
何首乌饮对雌性衰老大鼠肾脏皮质bcl-2/bax的影响
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1. 北京市大兴区亦庄医院 100176;
2. 中央财经大学校医院 100081;
3. 河北医科大学组胚教研室 050017;
4. 河北师范大学药物研究所 050016 -
DOI：
The Effect of Heshouwuyin on the Expression of bcl-2 and bax in Renal Cortex of Aging Famale Rats
摘要/Abstract
探讨中药何首乌饮对雌性衰老大鼠肾皮质bcl-2和bax表达的影响。选用50只雌性SD大鼠随机分为5组，即阴性对照组、模型组及何首乌饮高、中、低剂量组，每组10只。D半乳糖连续腹腔注射制作亚急性衰老大鼠模型。采用SP免疫组化法分别检测肾皮质组织中bcl-2和bax的表达情况。结果显示:1)模型组大鼠肾皮质bcl-2表达低于阴性对照组(P<0.05)，何首乌饮高剂量组与模型组比较表达增强(P<0.05)。2)模型组大鼠肾皮质bax表达明显高于阴性对照组(P<0.05)，何首乌饮各剂量组与模型组比较表达减弱(P<0.05)。研究提示何首乌饮可以通过上调bcl-2和下调bax表达，抑制肾皮质细胞凋亡，从而起到一定的延缓衰老作用。
To observe the effects of traditional Chinese medicine Heshouwuyin on the expression of bcl22 and bax in renal cortex of aging rats. 50 female SD rats were applied in this study and randomised into 5 groups:neg2 ative control group ,aging model group , Heshouwuyin groups (high ,middle and low dose group) ,10 rats per group. The sub-acute aging model rats were made by injecting D2gal into abdom inal cavity continuously. Using SP immunohistochemistry to observe the expression of bcl-2 and bax in renal cortex. Results:1) The expression of bcl-2 in model groups was weaker than that of negative control group(P<0.05),the bcl-2 expression was stronger in Heshouwuyin high dose group than that of model group (P<0.05).2) The expression of bax in model group was stronger than that of negative control group (P<0.05). The expression of bax in Hes2 houwuyin groups was weaker than that of model group (P<0.05). Traditional Chinese medicine Heshouwuyin could increase bcl-2 expression and decrease bax expression in renal cortex of aging rats obviously ,and had fairlyantiaging effects.