期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
抗菌肽Protegrin基因的定点诱变改良研究
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1. 河北师范大学 生命科学学院, 河北 石家庄 050016;
2. 河北省环境保护局, 河北 石家庄 050051 -
DOI：
Study on Improvement and Site-directed Mutagenesis of Antibacterial Peptide Protegrin
摘要/Abstract
通过对抗菌肽Protegrin成熟肽的氨基酸组成和作用机理分析,并根据已报道的Protegrin基因序列设计引物,PCR扩增获得定点诱变的突变体基因pg-g,其编码蛋白的末端相对于野生型增加1个非极性氨基酸Gly.利用表达载体pET-28a将突变体基因pg-g转化到大肠杆菌BL21(DE3),IPTG诱导表达后,经SDS-PAGE检测表明目的蛋白得到成功表达.通过对表达产物进行抑菌实验发现,定点突变基因pg-g表达产物对大肠杆菌的抑菌效果有明显提高.
According to the reported sequences of the mature peptide of protegrin and its gene, primers were designed to mutate its protein by adding a nonpolar amino acid(Gly)to its end. The mutant type protegrin gene pg-g was amplified by PCR. Then the pg-g gene was ligated with the expression vector pET-28a and transferred into the E. coli BL21(DE3). After inducing by IPTG and detecting by SDS-PAGE, the target protein was ex pressed successfully. Results of antibacterial experiment indicated that the expression product of pg-g gene ex hibited a higher antibacterial activity on Escherichia coli than its wild type.