期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
大肠杆菌K88ac, ST1, LTB基因融合
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1. 吉林化工学院 环境与生物工程学院, 吉林 吉林 132022;
2. 大连大学 医学院, 辽宁 大连 116622 -
DOI：
Gene Fusion of Enterotoxigenic Escherichia coli K88ac Gene, ST1 Gene and LTB Gene
摘要/Abstract
利用PCR技术,从大肠杆菌C83902质粒中扩增出K88ac基因,再从含ST1-LTB融合基因的重组质粒pXSLT1中扩增出ST1-LTB融合基因,构建了含K88ac-ST1-LTB融合基因表达质粒的重组菌株BL21(DE3)(pET-28KSL).经酶切鉴定和序列测定证实,构建的重组质粒pET-28KSL含有K88ac-ST1-LTB融合基因,且基因序列和阅读框架正确.经ELISA检测,重组菌株表达的K88ac-ST1-LTB融合蛋白能够被ST1,LTB单抗和K88ac抗体识别,表明已成功构建了K88ac-ST-LT融合基因,并实现了在重组大肠杆菌中的表达.
With the technology of PCR, K88ac gene and ST1-LTBfusion genes were amplified respectively from the plasmid of Escherichia coli C83902 and the recombinant plasmid pXSLT1. T he recombinant plasmid pET 28-KSL containing fusion gene K88ac ST1-LTB was obtained and transformed into Escherichia coli BL21(DE3). The recombinant plasmid pET 28-KSL contained the fusion gene K88ac -ST1-LTB which had correct se? quence and ORF by identification of endonuclease-digesting and sequence analysis. The fusion proteins K88ac? ST1-LTB were expressed in the recombinant strain BL21(DE3)(pET 28-KSL). By ELISA method, the ex- pressed protein could be recognized by MAb of ST1,LTBand K88ac antibodies. The result indicated that the fu? sion gene K88ac- ST1-LTBof enterotoxigenic Escherichia coli was successfully constructed and expressed in Es cherichia coli.