期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Determination of Diphenyl Methyl Acid Isomers by HPLC
摘要/Abstract
建立了3位和4位联苯甲酸同分异构体混合物的高效液相色谱法(HPLC)含量测定.色谱柱为Symmetry C18,检测波长254nm,流动相为甲醇-磷酸二氢钾(1.2mol/L),流速1mL/min.3-联苯甲酸的线性范围是48~720mg/L,4-联苯甲酸线性范围是21~685 mg/L;最低检出质量浓度均为0.01 mg/L,相关系数分别为0.9992和0.9998;平均回收率分别是99.4%和98.8%(n=6),RSD分别为0.87%和0.93%.
A HPLC method for determination of the isomeric compounds of 3 -diphenyl methyl acid and 4 - diphenyl methyl acid is established. The analytical column is Symmetry C18(150 mm @ 4. 6 mm, 5 Lm). The mobile phase consists of a mixture of methanol and 1. 2 mol/ L potassium dihydrogen phosphate with a flow rate of 1.0 mL/ min. The detection wavelength is 254 nm. The standard curve of 3 -diphenyl methyl acid and 4 -diphenyl methyl acid are linear in the range of 48~ 720 mg/ L and 21~ 685mg/ L(R= 0. 999 2 and R= 0. 9998)respectively. T he two lowest limits of the detections are 0. 01 mg/ L. The average recoveries are 99. 4 % and 98. 8%(n= 6)respectively. T he RSD are 0. 87 % and 0. 93 % respectively.