For a long time,the research for intestine iron metabolism mainly focused on the intestinal absorp-tivecell,so the role of the intestinal microvascular endothelial cells in iron absorption and transportin is still un-known.So microvascular was separated from the duodenum of SD rat through a method of two-enzyme digestion and different pore size filter.Then the comparable pure endothelial cell was gotten by the in vitro culture of the microvascular.And then iron transporter was detected for the first time associated protein divalent metal trans- porter1,feerroportin1 and Tf r1expression in the intestinal microvascular endothelial cells through western-blot,which will greatly enrich the theory of iron metabolism and provide theory and application supportion for the fur-ther iron metabolism and iron related diseases research.