期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
The Effect of Lycopene on the Differentiation of Osteoclast Induced from Bone Marrow in MiceThe Effect of Lycopene on the Differentiation of Osteoclast Induced from Bone Marrow in Mice
摘要/Abstract
建立由骨保护素配体(RANKL)和巨噬细胞集落刺激因子(M-CSF)共同细胞因子的小鼠破骨细胞骨髓诱导体系,将不同浓度的番茄红素作用于破骨细胞.受试细胞分为对照组、番茄红素低剂量组(10-8mol/L)、番茄红素中剂量组(10-7mol/L)和番茄红素高剂量组(10-6mol/L),并设立无细胞空白对照组.7d后取细胞玻片进行抗酒石酸酸性磷酸酶(TRAP)染色,观察破骨细胞并计数;取骨片进行甲苯胺蓝染色,光镜下统计骨吸收陷窝面积;测量TRAP活性以及破骨细胞表面NF-κB活化受体(RANK)mRNA表达量.诱导培养的破骨细胞形态特征明显;番茄红素中、高剂量组在细胞数量、TRAP活性、骨吸收陷窝面积上与对照组相比有明显统计学差异(p<0.05);番茄红素各剂量组在(RANK)mRNA表达量上与对照组相比有明显统计学差异(p<0.05),且呈量效关系.研究表明番茄红素可以抑制体外培养的破骨细胞分化.
The effect of lycopene on the differentiation of osteoclast was induced from bone marrow in vitro.Mononuclear cells of mice bone marrow were incubated with DMEM containing macrophage colony stimulating factor (M-CSF) and receptor activator of NF-JB ligand (RANKL).The culture was treated with lycopene of different concentrations (10-8,10-7,10-6mol/L).On the 7d,the culture cells were fixed and were stained for tartate-resistant acid phossphatase (TRAP).The formation of osteoclasts was quantified by counting the number of TRAP mult inuclear cells.The percentage of bone resorbed surface,TRAP activity and the expression of receptor activator of NF-JB (RANK) mRNA were analyzed.The appearance of reduced mice osteoclast swas typ-ical.Compared with the control group,the number of TRAP mult inuclear cells,TRAP activity and the percentage of bone surface in lycopene middle-dose group and lycopene high-dose group signif icantly decreased (pp<0.05).But the expression of RANK mRNA significantly increased(pp<0.05) by being treated in different dose of lycopene.Lycopene can inhibit osteoclasts differen tiation in vitro.