期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
链霉菌S506活菌制剂发酵条件的筛选与优化
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1. 河北省农林科学院遗传生理研究所, 河北石家庄050051;
2. 河北农业大学食品科技学院, 河北保定071000;
3. 河北师范大学生命科学学院, 河北石家庄050016 -
DOI：
Screening and Optimization of the Fermentation Conditions of Biogen Streptomyces S506
摘要/Abstract
对链霉菌S506活菌制剂的发酵条件进行了单因素研究,通过正交试验设计对该菌株的培养基进行了筛选.确定了液体发酵最佳培养基配方为麸皮40g/L,玉米粉40 g/L,甘油10mL/L,黄豆饼粉10g/L;最佳发酵工艺参数为温度35℃,初始pH为6∼8,500 mL三角瓶的装液量为100mL,转速150r/min,发酵周期96 h.经液-固发酵制得固体活菌菌剂,室温下保存1.5a,活菌数量仍达到40亿个/g以上,为大规模培养S506菌提供可靠的基础数据.
The fermenting conditions of Strep tomyces S506 were studied,the culture medium and culture condition for Strep tomyces which produced ant ibiotic were optimized by orthogonal design.The most optimal fermentation medium was determined,which was composed of 40g/L bran,40g/L corn flour,10mL/L glycerin and 10g/L soybean cake powder.The best fermentation parameters were as follows:35 eof temperature,initial pH 6∼ 8,100 mL fermentation liquid per 500 mL of conic bot tle,150r/min,96h fermentation period.The live fungus solid preparation was prepared by absorbing the fermentation liquid with solid carrier.The number of biogen Streptomyces S506 in the solid preparation could still reach more than 4 billion after 18 months of storage at room temperature.This provides some reliable basic data for a large scale cult ivation of S506 stain.