期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Effect on the Activity of Cholesterol Oxidase by Different MutationMethods
摘要/Abstract
以青霉TS406Y(Penicillum sp.)为诱变出发菌株,评价了硫酸二乙酯(DES)、亚硝基胍(NTG)和甲基磺酸乙酯(EMS)对该菌株的诱变效应,发现在常规剂量内达到70%致死率时所需要的时间分别为23,3,90 min.根据使用方便和安全原则,进行了青霉TS406Y的DES诱变和选育,获得了胆固醇氧化酶活性提高84.5%的突变菌株D5,其胆固醇氧化酶活性达到了1240 U/L,连续传代6次,发现D5菌株具有遗传稳定性.另外还发现,利用紫外线(UV)和EDS对单因素诱变后获得的胆固醇氧化酶高产菌株进行复合诱变,94%的突变菌株为负变菌株.
Mutated effect was determined of mutagenic agent which were diethyl sulfate,N-Methy-lN-n-itroso-N-nit roguanidine and Methanesulfonic acid ethylester,when the Penicillum sp.TS406Y was treated for 23,3,90 min by above mutagenic agent respect ively,70 % lethal rate was gotten.Cholesterol-deg rading mutant D5 was obtained from Penicillum sp.TS406Y by treatment with diethyl sulfate.The activity of cholesterol oxi-dase of strain D5,which was 1240 U/L,and was 84.5% higher than TS406Y.Meanwhile the results showed that 94% negative mutants were obtained by the combined treatment with ult raviolet and diethyl sulfate.