期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
MDV中meq基因对CEF细胞chTERT,chTR表达影响的研究
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1. 吉林大学军需科技学院, 吉林长春130062;
2. 吉林大学人兽共患病研究所, 吉林长春130062;
3. 吉林大学畜牧兽医学院, 吉林长春130062 -
DOI：
Study on the Effect of Expression Level of chTERT and chTR in Chicken Embryo Fibroblast by meq Gene
摘要/Abstract
利用TaqMan探针技术,采用实时荧光相对定量RT-PCR方法检测了MDV中meq基因对鸡胚成纤维细胞(CEF)的端粒酶催化亚单位基因(chTERT)、端粒酶RNA亚基(chTR)表达水平的影响,并用TRAP法测定了端粒酶活性,用流式细胞仪检测了细胞周期的变化.实验结果表明,转染48 h后chTERT表达水平为转染后72h的16倍,chTR表达水平在转染前后基本不变;转染48h后CEF细胞的相对端粒酶活性是转染72h后的12倍;在转染后72hS期细胞的百分比较未转染细胞显著增加.
ch TER T and chTR expression level in chicken embryo fibroblasts(CEF)were detected by Realtime relatively quantitative PCR method which was transfected by meq gene,through the TaqMan quantitative testing technique and the telomerase activity was detected with T RAP method and cell cycle changes also detected by flow cytometer.The results showed that the ex pression level of chTERT of 48h transfection was 16 times higher than those in 72h transfection while the expression level of ch TR hadn't changes af ter transfection.The telomerae activity of 48h transfection was 16 times hig her than those in 72h transfection.Flow Cy tometry was used to distinguish cell cycle phases and it was found that meq cause cell cycle arrest in S phase after 72h transfection.