期刊信息
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- 刊名: 河北师范大学学报(自然科学版)Journal of Hebei Normal University (Natural Science)
- 主办: 河北师范大学
- ISSN: 1000-5854
- CN: 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
从土壤中快速提取纯化DNA方法的建立
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1. 华北制药集团新药研究开发有限责任公司微生物药物国家研究工程中心, 河北石家庄050015;
2. 河北师范大学生命科学学院, 河北石家庄050016 -
DOI:
Simple and Rapid Method of Extracting and Purifying DNA from Soil
摘要/Abstract
摘要:
比较了从土壤中提取及纯化DNA的不同方法,确立了TENS-乙酸铵提取方法和PVPP柱层析的纯化方法.结果显示:TENS-乙酸铵提取方法对不同土壤均有较好的提取效果,DNA片段长度在23.1kb以上,且无明显降解;PVPP柱层析的纯化方法比胶回收纯化方法的DNA回收效率高、质量好,可用于PCR扩增.
Abstract:
Different methods were studied and compared to ext ract and purify DNA f rom the soil ,high puri-ty and quality DNA can be yielded by the TENS-ammonium acetate method containing f reeze-thaw cycles,bead2beating procedure.The size of DNA was above23.1kb with no obvious degradation.The PVPP spin column chromatography method shows high yield and quality than the Agarose Gel DNA Purification Kit.
关键词
Key words:
soil microbiology
;
DNA extraction
;
DNA purification
;
PCR