期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
Progress in Studies of Taxol Biosynthesis and Taxol Biosynthetic Enzymes
摘要/Abstract
紫杉醇的生物合成过程已基本阐明,其合成以牛儿基牛儿基二磷酸为前体约需20步酶促反应,近一半的相关酶基因已得到克隆与表达.综述了紫杉醇生物合成及生物合成途径中9个酶基因的克隆和表达方面的研究进展,并指出随着紫杉醇生物合成分子生物学研究的不断深入,利用分子生物技术大规模生产紫杉醇将为期不远.
The path of Taxol biosynthesis from geranylgeranyl diphosphate consists of about 20 enzy-matic steps, which has been elucidated approximately. Recent advances on T axol biosynthesis and the cloning and expression of the 9 genes encoding taxadiene synthase, cytochrome P450 taxdien 5 alpha-hy-droxylase, cytochrome P450 taxane 10 beta2hydroxylase, cytochrome P450 taxane 13 alpha2hydroxylase,taxadien25 alpha-ol2O2acetyl transferase, taxane 2 alpha-O-benzoyltransferase, 10-deacetylbaccatin- ó2102O2 acetyl transferase, 32amino232phenylpropanoyltransferase and 3c2N2debenzoyl22c2deoxytaxol N2benzoyltrans2 ferase were summarized respectively. The production of Taxol on large scale by biotechnology will become true recently with the development of studies on Taxol biosynthesis in molecular biology.