Cytoplasmic Ca²⁺ play an important role in pollen germination,the pathway through which apoplast Ca²⁺ enter pollen cells is a main topic in research about plant reproduction physiology.The effect of calcium channel blockers on Lilium daviddi pollen germination was investigated.The results showed that germinating pollens were adsorbing Ca²⁺ from extracellular medium.Blockers which mainly affect the activity of voltage-dependent calcium channel,including inorganic blockers such as La³⁺,Al³⁺ and Gd³⁺ and organic blockers such as verapamil,nifedipine and diltiazem remarkably inhibit pollen germination in a dose dependent manner,showing that voltage dependent calcium channel may be a main calcium influx pathway through which extracellular Ca²⁺ influx into germinating pollen cell.