期刊信息
- 刊名: 河北师范大学学报(自然科学版)Journal of Hebei Normal University (Natural Science)
- 主办: 河北师范大学
- ISSN: 1000-5854
- CN: 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
钙调素通过HSP70调控热激转录因子的活性
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1. 河北省农林科学院遗传生理研究所, 河北石家庄 050051;
2. 河北师范大学生命科学学院, 河北石家庄 050016 -
DOI:
Calmodulin Regulates Activity of HSF Through HSP70
摘要/Abstract
摘要:
凝胶阻滞分析结果显示,70kD的热激蛋白(HSP70)抑制热激转录因子(HSF)的DNA结合活性;利用免疫共沉淀法在细胞提取液中检测到了CaM-HSP70复合物的存在,并且热激后CaMHSP70复合物含量增加.表明钙调素(CaM)调控HSF活性可能是通过与HSP70结合起作用.
Abstract:
With gel mobility shift assay,it was found that DNA-binding activity of HSF was repressed by HSP70.It was also found that the CaM-HSP70 complex was existed in cytoplasmic extract by co-immunoprecipetation experiment,and concentration of CaM-HSP70 complex increased after heat shock.The results provide proofs for regulation of CaM on activity of HSF likely through binding to HSP70.Maize was used as experimental material.