期刊信息

  • 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
  • 主办： 河北师范大学
  • ISSN： 1000-5854
  • CN： 13-1061/N
  • 中国科技核心期刊
  • 中国期刊方阵入选期刊
  • 中国高校优秀科技期刊
  • 华北优秀期刊
  • 河北省优秀科技期刊

协同采编系统

友情链接

单宁酸检测方法的研究进展:从传统技术到纳米酶基传感器

  • （河北师范大学 生命科学学院，河北 石家庄 050024)
  • DOI： 10.13763/j.cnki.jhebnu.nse.202604011

Research progress in tannic acid detection:from traditional techniques to nanozyme-based sensors

摘要/Abstract

摘要：

单宁酸(tannic acid,TA)作为一种广泛存在于植物中的多酚类化合物，在食品、医药和工业领域具有重要的应用，但其过量摄入或排放会对人体健康和生态环境造成危害.本文系统综述了近年来单宁酸检测方法的研究进展，从传统检测方法(分光光度法、电化学法和色谱法)的原理与局限性出发，详细讨论了基于纳米酶的传感器在单宁酸检测中的优势，包括金属氧化物纳米酶、碳基纳米酶和复合纳米酶等体系的构建及其在比色、荧光和电化学检测中的应用.此外，汇总了双信号检测单宁酸的策略以及应用案例.最后，对单宁酸检测技术的发展趋势进行了展望，并指出高选择性、高灵敏度、便携化和智能化将是未来的重要研究方向.

Abstract：

Tannic acid(TA),a polyphenolic compound widely found in plants,holds significant applications in food,pharmaceuticals,and industrial fields.However,its excessive intake or discharge can pose risks to human health and ecosystems.This review systematically summarizes recent advances in TA detection methods,with a focus on novel nanozyme-based techniques.Starting from the principles and limitations of traditional methods—such as spectrophotometry,electrochemical assays,and chromatography—the review elaborates on the advantages of nanozyme-based sensors for TA detection.These include the construction of metal oxide nanozymes,carbon-based nanozymes,and composite nanozyme systems,as well as their applications in colorimetric,fluorescent,and electrochemical detection.Furthermore,the dual-signal detection strategies for TA and their application cases are also introduced.Finally,the future development trends of TA detection technology are prospected,with high selectivity,high sensitivity,portability,and intelligentization as critical research priorities.

关键词

关键词： 单宁酸； 纳米酶； 传感器； 检测
Key words： tannic acid ; nanozyme ; sensor ; detection

参考文献 60

  • [1] Sharma K,Kumar V,Kaur J,et al.Health effects,sources,utilization and safety of tannins:a critical review[J].Toxin Reviews,2021,40(4):432-444.DOI:10.1080/15569543.2019.1662813.
  • [2] Raj M A,Revin S B,John S A.Synthesis,characterization and modification of functionalized pyrimidine stabilized gold nanoparticles on ITO electrode for the determination of tannic acid[J].Bioelectrochemistry,2013,89:1-10.DOI:10.1016/j.bioelechem.2012.08.003.
  • [3] Liang T,Huang Y L,Yang L,et al.Hollow Mn/Co-MOF as a powerful oxidase-like nanozyme for detection of total antioxidant capacity and black tea fermentation degree[J].Small,2025,21(12):2411275.DOI:10.1002/smll.202411275.
  • [4] Zhu X Y,Chen C,Che D,et al.A high oxidase-like activity,bimetallic single-atom nanozyme FeCe/NC prepared by FeCe-ZIF-8 approach for sensing tannic acid in tea[J].Food Chemistry:X,2024,23:101552.DOI:10.1016/j.fochx.2024.101552.
  • [5] Zhang Y,Qin H W,Peng D N,et al.Au confined covalent organic frameworks nanoenzyme integrated with sodium alginate microsphere for portable colorimetric tannic acid detection[J].International Journal of Biological Macromolecules,2025,308:142556.DOI:10.1016/j.ijbiomac.2025.142556.
  • [6] Wu C H,Qin Z Y,Liu Y X,et al.Amorphous iron-catecholates featuring efficient peroxidase-like activity for quick colorimetric detection of tannic acid[J].LWT,2024,197:115896.DOI:10.1016/j.lwt.2024.115896.
  • [7] Suc L,Rigou P,Mouls L.Detection and identification of oxidation markers of the reaction of grape tannins with volatile thiols commonly found in wine[J].Journal of Agricultural and Food Chemistry,2021,69(10):3199-3208.
  • [8] Ahmed G H G,Laío R B,Calzón J A G,et al.Fluorescent carbon nanodots for sensitive and selective detection of tannic acid in wines[J].Talanta,2015,132:252-257.DOI:10.1016/j.talanta.2014.09.028.
  • [9] Piovesan J V,Santana E R,Spinelli A.A carbon paste electrode improved with poly(ethylene glycol) for tannic acid surveillance in beer samples[J].Food Chemistry,2020,326:127055.DOI:10.1016/j.foodchem.2020.127055.
  • [10] Yang H,He L,Pan S,et al.Nitrogen-doped fluorescent carbon dots for highly sensitive and selective detection of tannic acid[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2019,210:111-119.DOI:10.1016/j.saa.2018.11.029.
  • [11] Xu X K,Li W Q,Jing B,et al.A highly selective and sensitive Tb-MOF sensor for the fluorescence detection of tannic acid in beverages[J].Microchemical Journal,2024,202:110845.DOI:10.1016/j.microc.2024.110845.
  • [12] Dai Long V U,Ertek B,Dilgin Y,et al.Voltammetric determination of tannic acid in beverages using pencil graphite electrode[J].Czech Journal of Food Sciences,2015,33(1):72-76.DOI:10.17221/221/2014-cjfs.
  • [13] Rai P,Mehrotra S,Sharma S K.Development of a paper-based chromogenic strip and electrochemical sensor for the detection of tannic acid in beverages[J].LWT,2022,169:113999.DOI:10.1016/j.lwt.2022.113999.
  • [14] Lerdpiriyaskulkij N,Mathaweesansurn A,Monvisade P,et al.Aggregated gallic acid-modified platinum nanoparticles as colorimetric sensor for tannic acid detection in beverages based on displacement phenomenon[J].Microchemical Journal,2024,205:111201.DOI:10.1016/j.microc.2024.111201.
  • [15] Arul P,Nandhini C,Huang S T,et al.Development of water-dispersible Dy(Ⅲ)-based organic framework as a fluorescent and electrochemical probe for quantitative detection of tannic acid in real alcoholic and fruit beverages[J].Analytica Chimica Acta,2023,1274:341582.DOI:10.1016/j.aca.2023.341582.
  • [16] Liu J Y,Lv Y T,Zhou C Y,et al.Dual-signal determination of tannic acid based on DNA modified copper manganese oxide nanosheets and strong fluorescence copper doped silicon quantum dots[J].Microchemical Journal,2025,208:112539.DOI:10.1016/j.microc.2024.112539.
  • [17] Kapolou A,Karantonis H C,Rigopoulos N,et al.Association of mean daily polyphenols intake with mediterranean diet adherence and anthropometric indices in healthy greek adults:a retrospective study[J].Applied Sciences,2021,11(10):4664.DOI:10.3390/app11104664.
  • [18] Sistani S,Shekarchizadeh H.Fabrication of fluorescence sensor based on molecularly imprinted polymer on amine-modified carbon quantum dots for fast and highly sensitive and selective detection of tannic acid in food samples[J].Analytica Chimica Acta,2021,1186:339122.DOI:10.1016/j.aca.2021.339122.
  • [19] Ren A X,Zhang W Q,Thomas H G,et al.A tannic acid-based medical food,cesinex,exhibits broad-spectrum antidiarrheal properties:a mechanistic and clinical study[J].Digestive Diseases and Sciences,2012,57(1):99-108.DOI:10.1007/s10620-011-1821-9.
  • [20] Sun M M,Wang L L,Zhuo Y,et al.Multi-enzyme activity of MIL-101 (Fe)-derived cascade nano-enzymes for antitumor and antimicrobial therapy[J].Small,2024,20(17):2309593.DOI:10.1002/smll.202309593.
  • [21] Gülçini,HuyutZ,Elmastaş M,etal.Radicalscavengingandantioxidantactivityoftannicacid[J].ArabianJournalof Chemistry,2010,3(1):43-53.DOI:10.1016/j.arabjc.2009.12.008.
  • [22] Qiao C L,Wang X R,Luo H B,et al.From discoloration to color enhancement:a novel approach to high-concentration tannic acid detection based on VCo-N-C bimetallic nanozyme[J].Food Chemistry,2025,481:144021.DOI:10.1016/j.foodchem.2025.144021.
  • [23] Wang X H,Liu W L,Ma H Y,et al.Glutathione-mediated copper sulfide nanoplatforms with morphological and vacancy-dependent photothermal catalytic activity for multi-model tannic acid assays[J].Journal of Colloid and Interface Science,2024,670:460-472.DOI:10.1016/j.jcis.2024.05.128.
  • [24] Guo X Y,Wu L L,Ma L Z,et al.White nanozymes with enhanced alkaline phosphatase-mimicking activity and selective inhibition effect:enzyme-free colorimetric test strip of pesticide[J].Advanced Functional Materials,2026,36(11):e09324.DOI:10.1002/adfm.202509324.
  • [25] Chung K T,Wong T Y,Wei C I,et al.Tannins and human health:a review[J].Critical Reviews in Food Science and Nutrition,1998,38(6):421-464.DOI:10.1080/10408699891274273.
  • [26] Chen X,Huang Z H,Ji Z Y,et al.Efficient treatment of pure terephthalic acid wastewater with Na2S2O8 based on thermal activation[J].Environmental Technology & Innovation,2020,19:100897.DOI:10.1016/j.eti.2020.100897.
  • [27] Shahidi F,Ambigaipalan P.Phenolics and polyphenolics in foods,beverages and spices:antioxidant activity and health effects:a review[J].Journal of Functional Foods,2015,18:820-897.DOI:10.1016/j.jff.2015.06.018.
  • [28] Feng S L,Tang A N,Jiang J H,et al.Spectrofluorimetric determination of tannins based on their activative effect on the Cu(Ⅱ) catalytic oxidation of rhodamine 6G by hydrogen peroxide[J].Analytica Chimica Acta,2002,455(2):187-191.DOI:10.1016/S0003-2670(01)01602-6.
  • [29] Sharma O P,Bhat T K,Singh B.Thin-layer chromatography of gallic acid,methyl gallate,pyrogallol,phloroglucinol,catechol,resorcinol,hydroquinone,catechin,epicatechin,cinnamic acid,p-coumaric acid,ferulic acid and tannic acid[J].Journal of Chromatography A,1998,822(1):167-171.DOI:10.1016/S0021-9673(98)00490-7.
  • [30] Vu D L,Ertek B,ˇCervenka L,et al.Determination of tannic acid using silica gel modified carbon paste electrode[J].International Journal of Electrochemical Science,2013,8(7):9278-9286.DOI:10.1016/S1452-3981(23)12967-1.
  • [31] Xue P P,Zhuang H L,Shao S J,et al.Engineering biodegradable hollow silica/iron composite nanozymes for breast tumor treatment through activation of the ″ferroptosis storm″[J].ACS Nano,2024,18(37):25795-25812.
  • [32] Shu R,Liu S J,Zhao C,et al.Two birds with one stone:gold ″bridging″ both nanozyme and immunosensor for sensitive double-response detection of T-2 toxin[J].Chemical Engineering Journal,2024,481:148473.DOI:10.1016/j.cej.2023.148473.
  • [33] Wu P,Ding P,Ye X S,et al.One-pot synthesized Cu/Au/Pt trimetallic nanoparticles as a novel enzyme mimic for biosensing applications[J].RSC Advances,2019,9(26):14982-14989.
  • [34] Nakamura T,Coichev N,Moya H D.Modified CUPRAC spectrophotometric quantification of total polyphenol content in beer samples using Cu(Ⅱ)/neocuproine complexes[J].Journal of Food Composition and Analysis,2012,28(2):126-134.DOI:10.1016/j.jfca.2012.07.012.
  • [35] Rodríguez H,de las Rivas B,Gómez-Cordovés C,et al.Degradation of tannic acid by cell-free extracts of Lactobacillus plantarum[J].Food Chemistry,2008,107(2):664-670.DOI:10.1016/j.foodchem.2007.08.063.
  • [36] Arapitsas P,Menichetti S,Vincieri F F,et al.Hydrolyzable tannins with the hexahydroxydiphenoyl unit and the m-depsidic link:HPLC-DAD-MS identification and model synthesis[J].Journal of Agricultural and Food Chemistry,2007,55(1):48-55.
  • [37] Xu W H,Han E H,Wang Z Y.Effect of tannic acid on corrosion behavior of carbon steel in NaCl solution[J].Journal of Materials Science & Technology,2019,35(1):64-75.DOI:10.1016/j.jmst.2018.09.001.
  • [38] Han L,Zhang H J,Chen D Y,et al.Protein-directed metal oxide nanoflakes with tandem enzyme-like characteristics:colorimetric glucose sensing based on one-pot enzyme-free cascade catalysis[J].Advanced Functional Materials,2018,28(17):1800018.DOI:10.1002/adfm.201800018.
  • [39] Montini T,Melchionna M,Monai M,et al.Fundamentals and catalytic applications of CeO2-based materials[J].Chemical Reviews,2016,116(10):5987-6041.DOI:10.1021/acs.chemrev.5b00603.
  • [40] Fan X K,Yu H F,Zhang M P,et al.Oxygen defects enriched cerium oxide nanozyme for sensitive colorimetric detection of tannic acid[J].Particle & Particle Systems Characterization,2025,42(7):2400249.DOI:10.1002/ppsc.202400249.
  • [41] Kang X,Ren Y,Wang J,et al. A noble metal-enhanced Au@CuO heterostructure with multienzyme-mimicking activities for colorimetric detection of tannic acid[J].Analyst,2024,149(19):4889-4898.DOI:10.1039/D4AN01013B.
  • [42] Wu X P,Wang Z C,Liu Y L,et al.Polymeric schiff base assisted synthesis of Fe-N-C MFs single-atom nanozymes for discrimination and intelligent sensing of tannic acid[J].Chemical Engineering Journal,2023,468:143638.DOI:10.1016/j.cej.2023.143638.
  • [43] Liu C T,Ye F,Fu Y.Nanoscale light warriors:a review of carbon dot-based optical sensors for insecticide residue detection in food and ecosystems[J].Talanta,2026,296:128445.DOI:10.1016/j.talanta.2025.128445.
  • [44] Serag A,Abdelazim A H,Ramzy S,et al.A fluorescent sensor based on nitrogen-doped graphene quantum dots and molecularly imprinted polymers for selective and sensitive detection of meropenem in environmental and clinical matrices[J].Talanta,2026,296:128442.DOI:10.1016/j.talanta.2025.128442.
  • [45] Asif S,Morgan D,Sen K.Graphene quantum dots from pyrolytic condensation of aspartic acid and glycerol for ratiometric fluorosensing of lactic acid in cancer cells[J].Diamond and Related Materials,2025,157:112547.DOI:10.1016/j.diamond.2025.112547.
  • [46] Hou L M,Hu X L,Pang W Y,et al.A dual-channel fluorescent sensing system based on carbon dots and photoswitchable Pdots for sensitive detection of acetamiprid and carbendazim in food samples[J].Talanta,2026,296:128444.DOI:10.1016/j.talanta.2025.128444.
  • [47] Zhang Y,Zhao Z Y,Wu H.Sensitive fluorescence sensor for isoliquiritigenin determination based on N-doped carbon dots[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2025,343:126554.DOI:10.1016/j.saa.2025.126554.
  • [48] Fu Q J,Liang S,Su X G.Nanowire-like phosphorus modulated carbon-based iron nanozyme with oxidase-like activity for sensitive detection of choline and dye degradation[J].Talanta,2025,283:127169.DOI:10.1016/j.talanta.2024.127169.
  • [49] Li Y,Yang Y Q,Jiang Y M,et al.Detection of tannic acid exploiting carbon dots enhanced hydrogen peroxide/potassium ferricyanide chemiluminescence[J].Microchemical Journal,2020,157:105113.DOI:10.1016/j.microc.2020.105113.
  • [50] Liu X N,Zhang W T,Yang C Y,et al.Rapid and selective fluorometric determination of tannic acid using MoO3-x quantum dots[J].Microchimica Acta,2019,186(4):247.DOI:10.1007/s00604-019-3311-2.
  • [51] Yu B,Bai Y J,Gao W Q,et al.Colorimetric sensor array constructed based on bimetallic porphyrin-based metal organic framework nanozyme for the detection and recognition of tannic acid[J].Food Chemistry,2025,486:144592.DOI:10.1016/j.foodchem.2025.144592.
  • [52] Wang C Y,Huang P,You T,et al.1D MnO2 nanowires as active oxidase mimics in fabrication of colorimetric/photothermal dual-mode detection system for cysteine in soy sauce[J].Food Chemistry,2025,489:144950.DOI:10.1016/j.foodchem.2025.144950.
  • [53] Huang Y,Ji Y X,Wu M Y,et al.TA-TaTe2@lysozyme-based smartphone-integrated lateral flow biosensor for colorimetric and photothermal dual-mode bacterial label-free detection[J].Sensors and Actuators B:Chemical,2025,438:137779.DOI:10.1016/j.snb.2025.137779.
  • [54] Sun S J,Yang J Q,Yang G G,et al.Cyclodextrin reduced Fe(Ⅲ) nanozyme-based colorimetric and photothermal dual-mode assay for early monitoring meat freshness by sensitive detection of hypoxanthine[J].Food Chemistry,2025,480:143917.DOI:10.1016/j.foodchem.2025.143917.
  • [55] Zhou Z F,Li Y,Wen D Y,et al.A CRISPR-Cas13a powered electrochemical sensor based on reduced graphene oxide,polypyrrole and gold nanoparticles nanocomposites for PEDV detection[J].Bioelectrochemistry,2025,166:109019.DOI:10.1016/j.bioelechem.2025.109019.
  • [56] Li K R,Guo W S,Yu H,et al.A versatile strategy for constructing background fluorescence-free and dual-mode hydrogel nanosensor for sensitive detection of amikacin[J].Talanta,2026,296:128410.DOI:10.1016/j.talanta.2025.128410.
  • [57] Wang W T,Ren Y P,Guan B,et al.Flower-like ytterbium prussian blue analogue nanozyme with freeze-drying method for colorimetric/photothermal/smartphone triple-mode detection of thiosulfate[J].Biosensors and Bioelectronics,2025,290:117997.DOI:10.1016/j.bios.2025.117997.
  • [58] Liu B,Yin Y,Li Q W,et al.Dual-signal detection of tannic acid in red wines based on the peroxidase activity of carbon dots[J].Analytical Methods,2024,16(18):2948-2958.
  • [59] Tang R,Zhang C L,Liu B L,et al.Colorimetry/fluorescence dual-signal sensing system based on peptide nanotubes activating hemin and PPIX for sensitive detection of tannic acid[J].Talanta,2025,287:127663.DOI:10.1016/j.talanta.2025.127663.
  • [60] Peng J S,Liu Y F,Cheng X,et al.Race nanoparticles with peroxidase-like activity for colorimetric and temperature detection of tannic acid[J].Microchemical Journal,2024,199:110205.DOI:10.1016/j.microc.2024.110205.

版权所有 2013-2021 河北师范大学学报编辑部 地址：河北省石家庄市南二环东路20号

邮编：050024 电话：0311-80786360 技术支持：载驰科技