期刊信息

  • 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
  • 主办： 河北师范大学
  • ISSN： 1000-5854
  • CN： 13-1061/N
  • 中国科技核心期刊
  • 中国期刊方阵入选期刊
  • 中国高校优秀科技期刊
  • 华北优秀期刊
  • 河北省优秀科技期刊

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基于ZIF-8负载荧光核酸适配体的黄曲霉毒素 B1高灵敏度检测方法

  • (1.河北省市场监督管理局专业技术能力提升中心，河北 石家庄 050000； 2.河北科技大学 化学与制药工程学院，河北 石家庄 050018)
  • DOI： 10.13763/j.cnki.jhebnu.nse.202603015

A highly sensitive detection method for aflatoxin B1 based on ZIF-8 loaded fluorescent DNA aptamer

摘要/Abstract

摘要：

黄曲霉毒素B1（aflatoxin B1,AFB1）是一类毒性极强的霉菌毒素，实现食品中AFB1的灵敏、准确检测具有重要意义.基于5-羧基四甲基罗丹明（TAMRA）标记的核酸适配体与金属有机框架ZIF-8构建了一种新型荧光适配体传感器，用于食品中AFB1的快速检测.在无AFB1时，核酸适配体通过静电或π-π堆积作用固定于ZIF-8表面，导致荧光淬灭；当存在AFB1时，核酸适配体与AFB1结合，并转变为内环结构，从ZIF-8表面脱离，荧光信号得以恢复.该传感器检出限低至1 nmol/L，对食品中常见干扰物质表现出良好的选择性.将该方法应用于玉米、黄豆、葵花籽实际样品中AFB1的检测，验证了其可靠性和实用性，为食品中AFB1的快速筛查提供了一种简便高效的技术手段.

Abstract：

Aflatoxin B1(AFB1) is a class of extremely toxic fungal toxin,and it is of great significance to realize the sensitive and accurate detection of AFB1 in food.A novel fluorescent aptamer sensor for rapid detection of AFB1 in food was developed based on 5-carboxy tetramethylrhodamine(TAMRA) labeled aptamer and metal organic framework ZIF-8.In the absence of AFB1,the aptamer is immobilized on the surface of ZIF-8 through electrostatic or π-π stacking,resulting in fluorescence quenching.When AFB1 is present,the adapter binds to AFB1 and transforms into an inner ring structure,detaching from the surface of ZIF-8 and restoring the fluorescence signal.The detection limit of this sensor is as low as 1 nmol/L,with the good selectivity for common interfering substances in food.The method is applied to the detection of AFB1 in actual samples of corn,soybeans and sunflower seeds,showing its reliability and practicality,which provides a simple and efficient technical means for rapid screening of AFB1 in food.

关键词

关键词： 黄曲霉毒素B1 ; ZIF-8 ; 核酸适配体 ; 荧光传感器
Key words： aflatoxin B1 ; ZIF-8 ; aptamer ; fluorescent sensor

参考文献 23

  • [1] Lai Y T,Kandasamy K,Chen Y C.Magnetic graphene oxide-based affinity surface-assisted laser desorption/ionization mass spectrometry for screening of aflatoxin B1 from complex samples[J].Analytical Chemistry,2021,93(19):7310-7316.DOI:10.1021/acs.analchem.1c00878.
  • [2] Ayeni K I,Seki D,Pjevac P,et al.Biomonitoring of dietary mycotoxin exposure and associated impact on the gut microbiome in Nigerian infants[J].Environmental Science & Technology,2024,58(5):2236-2246.DOI:10.1021/acs.est.3c07786.
  • [3] Qin M,Cheng L,Li Y,et al.Disease burden contributed by dietary exposure to aflatoxins in a mountainous city in Southwest China[J].Frontiers in Microbiology,2023,14:1215428.DOI:10.3389/fmicb.2023.1215428.
  • [4] Annunziata L,Stramenga A,Visciano P,et al.Simultaneous determination of aflatoxins,T-2 and HT-2 toxins,and fumonisins in cereal-derived products by QuEChERS extraction coupled with LC-MS/MS[J].Analytical and Bioanalytical Chemistry,2017,409(21):5143-5155.DOI:10.1007/s00216-017-0462-z.
  • [5] Rodríguez-Carrasco Y,Moltó J C,Maes J,et al.Development of a GC-MS/MS strategy to determine 15 mycotoxins and metabolites in human urine[J].Talanta,2014,128:125-131.DOI:10.1016/j.talanta.2014.04.072.
  • [6] Irakli M N,Skendi A,Papageorgiou M D.HPLC-DAD-FLD method for simultaneous determination of mycotoxins in wheat bran[J].Journal of Chromatographic Science,2017,55(7):690-696.DOI:10.1093/chromsci/bmx022.
  • [7] Fan Y Y,Liu F J,He W Z,et al.Screening of multi-mycotoxins in fruits by ultra-performance liquid chromatography coupled to ion mobility quadrupole time-of-flight mass spectrometry[J].Food Chemistry,2022,368:130858.DOI:10.1016/j.foodchem.2021.130858.
  • [8] Dou X L,Wu G,Ding Z Y,et al.Construction of a nanoscale metal-organic framework aptasensor for fluorescence ratiometric sensing of AFB1 in real samples[J].Food Chemistry,2023,416:135805.DOI:10.1016/j.foodchem.2023.135805.
  • [9] Zhang J J,Li Z M,Zhao S C,et al.Size-dependent modulation of graphene oxide-aptamer interactions for an amplified fluorescence-based detection of aflatoxin B1 with a tunable dynamic range[J].Analyst,2016,141(13):4029-4034.DOI:10.1039/c6an00368k.
  • [10] Seok Y,Byun J Y,Shim W B,et al.A structure-switchable aptasensor for aflatoxin B1 detection based on assembly of an aptamer/split DNAzyme[J].Analytica Chimica Acta,2015,886:182-187.DOI:10.1016/j.aca.2015.05.041.
  • [11] Kuai H L,Zhao Z L,Mo L T,et al.Circular bivalent aptamers enable in vivo stability and recognition[J].Journal of the American Chemical Society,2017,139(27):9128-9131.DOI:10.1021/jacs.7b04547.
  • [12] Wang Z J,Luo Y,Xie X D,et al.In situ spatial complementation of aptamer-mediated recognition enables live-cell imaging of native RNA transcripts in real time[J].Angewandte Chemie International Edition,2018,130(4):984-988.DOI:10.1002/ange.201707795.
  • [13] Yang X S,Shi D M,Zhu S M,et al.Portable aptasensor of aflatoxin B1 in bread based on a personal glucose meter and DNA walking machine[J].ACS Sensors,2018,3(7):1368-1375.DOI:10.1021/acssensors.8b00304.
  • [14] 庄景果,过容利,侯文平,等.钛族金属对苯二甲酸框架材料对水溶液中染料和金属离子的吸附性能[J].河北师范大学学报(自然科学版),2021,45(3):290-297.DOI:10.13763/j.cnki.jhebnu.nse.202103010. Zhuang Jingguo,Guo Rongli,Hou Wenping,et al.ⅣB metal-terephthalic acid MOF for adsorption of dyes and metal ionic[J].Journal of Hebei Normal University(Natural Science),2021,45(3):290-297.
  • [15] Anderson S L,Boyd P G,Gladysiak A,et al.Nucleobase pairing and photodimerization in a biologically derived metal-organic framework nanoreactor[J].Nature Communications,2019,10:1612.DOI:10.1038/s41467-019-09486-2.
  • [16] 魏雨,李巧玲,武克忠,等.纳米微粒的性质及制备[J].河北师范大学学报(自然科学版),1998,22(2):243-246.DOI:10.13763/j.cnki.jhebnu.nse.1998.02.029. Wei Yu,Li Qiaoling,Wu Kezhong,et al.Character and preparation of nanoparticles[J].Journal of Hebei Normal University(Natural Science),1998,22(2):243-246.
  • [17] Pang F,He M Y,Ge J P.Controlled synthesis of Fe3O4/ZIF-8 nanoparticles for magnetically separable nanocatalysts[J].Chemistry-A European Journal,2015,21(18):6879-6887.DOI:10.1002/chem.201405921.
  • [18] Li Y,Jiang L L,Qiao Y X,et al.Yolk-shell magnetic composite Fe3O4@Co/Zn-ZIF for MR imaging-guided chemotherapy of tumors in vivo[J].New Journal of Chemistry,2021,45(9):4326-4334.DOI:10.1039/d0nj05723a.
  • [19] Sahu K P,Singh S,Singh A K,et al.Nanomaterials via ZIF-8:preparations,catalytic and drug delivery applications[J].Chemical Engineering Journal,2025,508:160663.DOI:10.1016/j.cej.2025.160663.
  • [20] Liang M M,Zhou W X,Zhang H F,et al.Tumor microenvironment responsive T1-T2 dual-mode contrast agent Fe3O4@ZIF-8-Zn-Mn NPs for in vivo magnetic resonance imaging[J].Journal of Materials Chemistry B,2023,11(19):4203-4210.DOI:10.1039/d3tb00068k.
  • [21] Li Y,Dong X,Wu T T,et al.Zirconium based metal-organic frameworks with aggregation-induced electrochemiluminescence for sensitive analysis of aflatoxin B1 by signal dual-amplification strategy[J].Chemical Engineering Journal,2024,500:157308.DOI:10.1016/j.cej.2024.157308.
  • [22] Yang Z Q,Qian Y,Yang F,et al.Investigating adsorption/desorption of DNA on ZIF-8 surface by fluorescently labeled oligonucleotides[J].Langmuir,2019,35(49):16290-16296.DOI:10.1021/acs.langmuir.9b02692.
  • [23] Jia Y M,Wu F,Liu P L,et al.A label-free fluorescent aptasensor for the detection of aflatoxin B1 in food samples using AIEgens and graphene oxide[J].Talanta,2019,198:71-77.DOI:10.1016/j.talanta.2019.01.078.

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