It was investigated the fixation of cellulase on chitosan where the double functional agent glutaric dialdehyde was used as a cross-liker and the deacylation of ploy-chitose is up to 93.66%.The concentration of cross linker,quantity of enzyme and offer fixing conditions were all considered.Meanwhile,some physics and chemistry characters of fixating enzyme,such as thermostability,the value of Michaelis constant the optimum temperature and pH were all studied.The results demonstrated that the higher deacylation of poly-chitose corresponds to the higher concentration of glutaral dehyde and correspondingly,larger quantity of fixation enzyme,the thermal stability of fixing enzyme were also increased.The fact that the most proper pH value shifted to the acidic direction was also observed.