期刊信息

  • 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
  • 主办： 河北师范大学
  • ISSN： 1000-5854
  • CN： 13-1061/N
  • 中国科技核心期刊
  • 中国期刊方阵入选期刊
  • 中国高校优秀科技期刊
  • 华北优秀期刊
  • 河北省优秀科技期刊

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基于碳点“开-关-开”荧光响应模式检测Hg²⁺和谷胱甘肽

  • (河北师范大学 化学与材料科学学院，石家庄 050024)
  • DOI： 10.13763/j.cnki.jhebnu.nse.202503011

Detection of Hg2+ and Glutathione Based on the "On-off-on" Fluorescence Response Mode of Carbon Dots

摘要/Abstract

摘要：

以抗坏血酸和尿素为原料，通过一步水热法合成了氮掺杂碳点（N-CDs），并采用各种表征手段对产物的形貌、荧光性能等进行了分析，探讨了其荧光响应机制.结果表明，所制备的N-CDs的平均粒径约为10 nm，荧光量子产率为25%，且具有较好的荧光稳定性.该N-CDs对Hg2+具有较高的选择性，其荧光猝灭程度与Hg2+的摩尔浓度(10~70 μmol/L)之间呈良好的线性关系(R2=0.997 39);谷胱甘肽(GSH)能够恢复N-CDs@Hg2+溶液的荧光强度，且体系荧光强度与GSH的摩尔浓度(10~50 μmol/L)之间呈良好的线性关系(R2=0.998 52)；该N-CDs对Hg2+和GSH的检出限较低，分别为0.004 1，0.027 6 μmol/L.开发了一种通过“开-关-开”荧光响应模式灵敏地检测Hg2+和GSH的新方法.

Abstract：

Nitrogen-doped carbon dots(N-CDs) were synthesized by one-step hydrothermal method using ascorbic acid and urea as raw materials.The morphology and fluorescence properties of the N-CDs were analyzed by various characterization technologies,and the fluorescence response mechanism was explored.The results showed that the average size of the N-CDs was about 10 nm with fluorescence quantum yield of 25% and good fluorescence stability.The N-CDs exhibited high fluorescent selectivity for Hg2+,and the fluorescence quenching degree of N-CDs had a good linear relationship(R2=0.997 39) with the molar concentration of Hg2+(10~70 μmol/L).The glutathione(GSH) restored the fluorescence intensity of N-CDs@Hg2+solution,and the fluorescence intensity showed a good linear relationship(R2=0.998 52) with the molar concentration of GSH(10~50 μmol/L).The lower detection limits of the N-CDs for Hg2+ and GSH are 0.004 1,0.027 6 μmol/L,respectively.A new and sensitive method for the detection of Hg2+ and GSH is developed by the fluorescence response mode of "on-off-on".

关键词

关键词： 碳点； 汞离子； 谷胱甘肽； 荧光探针
Key words： carbon dots ; Hg2+ ; glutathione ; fluorescent probe

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