期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
高效液相色谱法测定胶囊类保健食品中红景天苷和酪醇含量
- (1.天津科技大学 生物工程学院，天津 300457； 2.河北省药品医疗器械检验研究院，河北 石家庄 050200)
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DOI：10.13763/j.cnki.jhebnu.nse.202403012
Determination of Salidroside and Tyrosol in Capsule Health Food by HPLC
摘要/Abstract
建立了胶囊类保健食品中红景天苷和酪醇含量的测定方法.采用高效液相色谱法(HPLC)，以Thermo Scientific AcclaimTM 120 C18(250 mm×4.6 mm，5 μm)为色谱柱，0.01 mol/L乙酸铵溶液甲醇(V(乙酸铵溶液）∶V（甲醇）=80∶20)为流动相，流速1.0 mL/min，检测波长220 nm.结果表明，红景天苷的色谱峰面积与质量浓度（0.010 43~0.521 5 mg/mL）和酪醇的色谱峰面积与质量浓度（0.009 9~0.198 8 mg/mL）均呈良好线性关系，相关系数（r）分别为0.999 7，0.999 8.红景天苷和酪醇的平均加标回收率分别为100.17 %，100.06 %，RSD分别为1.05 %，1.61 %.该方法稳定可靠、切实可行、操作简便、结果准确，可用于胶囊类保健食品中红景天苷和酪醇的含量测定.
A method for the determination of salidroside and tyrosol in capsule health food was established.With Thermo Scientific AcclaimTM 120 C18(250 mm×4.6 mm，5 μm) as chromatographic column，0.01 mol/L ammonium acetate solution-methanol(volume ratio of 80∶20) as the mobile phase,flow rate of 1.0 mL/min，and detection wavelength of 220 nm,the HPLC results showed that there was a good linear relationship between the peak area and mass concentration(0.010 43~0.521 5 mg/mL for salidroside and 0.009 9~0.198 8 mg/mL for tyrosol),with the correlation coefficients(r) of 0.999 5 and 0.999 7,respectively.The average recovery rates of salidroside and tyrosol were 100.17 % and 100.06 %, with RSDs of 1.05 % and 1.61 %,respectively.This method is stable and reliable,practical and simple operation.Importantly,the result is accurate.It can be used for the determination of salidroside and tyrosol in capsule health food.
关键词
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