Encarsia sophia is an important natural enemy of Bemisia tabaci. It is a single parasitoid, in which females are alone endoparasitoid and males are autoparasitoid, also known as hyperparasitoid. The male and female bees are different in the host, parasitic way and development environment. Herein, for the first time, the whole process of the embryo development of E. sophia male and female bees was continuously observed and photographed by electron microscope, and the changes in the length of both male and female embryos were measured. The result showed that three layers of membranes occurred during the development of female embryos, and the external two layers are embryonic membrane (chorion) and extraembryonic membrane (trophamnion). The extraembryonic membrane can be seen under a microscope when the embryos developed to about 8 h, and disappeared until the female eggs hatched. In contrast, only two layers of membranes appeared during the development of male embryos, and extraembryonic membrane did not observed. There was a pedicel in the front of the male egg, and it disappeared when the male embryo began to hatch, while the pedicel of female embryo emerged at 2 h of development and disappeared at 24 h. The length of female embryos increased rapidhy from 0 h to 36 h (0.198~0.280 mm), whereas the length of male embryos showed minor change during this time (0.163~0.187 mm). Both male and female embryos hatched from 36 h to 48 h, and the body length obviously increased.