期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
β-环糊精敏化荧光法测定黄柏中盐酸小檗碱的含量
- (1.石家庄财经职业学院,河北 石家庄 050061; 2.河北师范大学 化学与材料科学学院,河北 石家庄 050024; 3.石家庄生产力促进服务中心,河北 石家庄 050090; 4.河北工业职业技术大学 环境与化学工程系,河北 石家庄 050091)
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DOI：10.13763/j.cnki.jhebnu.nse.202303004
Determination of Berberine Hydrochloride in Phellodendron by β-cyclodextrin Sensitized Fluorescence
摘要/Abstract
摘要：
采用β-环糊精敏化荧光法测定黄柏提取液中盐酸小檗碱的含量. 盐酸小檗碱是黄柏的主要提取物,与β-环糊精可以形成包合物,产生敏化荧光作用. 在7.50 mg/mL β-环糊精水溶液中,选择荧光激发波长(λex)345 nm,发射波长(λem)540 nm,工作曲线线性范围0.10～1.00 μg/mL,检出限1.33 ng/mL,测得黄柏中盐酸小檗碱的含量为1.56 %.此方法操作简单、准确,对中药中盐酸小檗碱的含量测定具有一定的参考价值.
Abstract：
The content of berberine hydrochloride in phellodendron extracting solution was determined by β-cyclodextrin sensitized fluorescence method. Berberine hydrochloride is the main extract of phellodendron,which can form inclusion complex with β-cyclodextrin to generate fluorescence sensitization.In 7.50 mg/mL β-cyclodextrin aqueous solution,the excitation wavelength(λex) and emission wavelength(λem) of the fluorescence detection were 345 and 540 nm,respectively. The linear range of the working curve was 0.10 to 1.00 μg/mL,and the detection limit was 1.33 ng/mL. The results showed that the content of berberine hydrochloride in phellodendron was 1.56 %.This method is simple and accurate,and has certain reference value for the determination of berberine hydrochloride in traditional Chinese medicine.
关键词
参考文献 18
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