河北师范大学学报—

利用生理学实验分析番茄幼苗主根对不同浓度油菜素甾醇（BRs）的响应情况，通过转录组测序寻找番茄幼苗根系在不同浓度BRs处理下的差异表达基因，利用GO与KEGG富集分析探究差异表达基因参与的信号通路. 结果发现，0. 5 nmol/L的表油菜素内酯（eBL，BRs的一种）促进主根伸长，而10 nmol/L的eBL显著抑制主根伸长. 转录组测序发现，与对照相比，10 nmol/L eBL处理共产生585个差异表达基因，其中271个上调和314个下调. 进一步的GO与KEGG富集分析表明，上调的差异基因主要与乙烯合成与代谢、植物激素信号转导和MAPK信号转导有关，暗示BRs可能与乙烯交叉互作调控高浓度BRs对植物主根生长的抑制. 研究结果为BRs参与植物根系发育调控提供了又一证据，也为番茄等农作物生产中科学使用BRs提供了强有力的支撑.

Abstract：

Physiological experiments were conducted to analyze the response of tomato seedlings to different concentrations of brassinosteroids (BRs), and transcriptome sequencing was performed to identify differentially expressed genes (DEGs) in tomato seedling treated with different concentrations of BRs. GO and KEGG enrichment analysis were employed to explore the signaling pathways involving DEGs. The physiological experiments showed that 0. 5 nmol/L eBL promoted primary root elongation, whereas 10 nmol/L eBL significantly inhibited primary root elongation. Transcriptome sequencing revealed that a total of 585 DEGs were identified in tomato seedlings treated with 10 nmol/L eBL, including 271 up-regulated genes and 314 down-regulated genes. Further GO and KEGG enrichment analysis showed that up-regulated differentially expressed genes were mainly involved in ethylene synthesis and metabolism, plant hormone signal transduction and MAPK signal transduction, suggesting that BRs may regulate plant root growth through the crosstalk with ethylene. The results would provide one more evidence that BRs are involved in the regulation of plant root development, and also provide strong support for the scientific use of BRs in the production of tomato and other crops.

关键词

关键词： 油菜素甾醇；主根伸长；差异表达基因；乙烯合成与代谢； MAPK信号转导

Key words： brassinosteroids (BRs) ; primary root elongation ; differentially expressed genes ; ethylene synthesis and metabolism ; MAPK signal transduction

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