期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
低温好氧反硝化菌的选育以及脱氮性能
- (深圳市长隆科技有限公司 迈葳生物事业部，广州 深圳 518116)
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DOI：10.13763/j.cnki.jhebnu.nse.202204010
Breeding and Denitrification Performance of Psychrotolerant-aerobic Denitrifying Bacteria
摘要/Abstract
通过接种某污水处理厂循环活性污泥工艺(cyclic sludge system，CASS)反应池出口活性污泥到培养基，经过对污泥驯化从中分离出1株低温好氧反硝化菌IL-2，温度 4 ℃.对IL-2菌进行常规的生理生化鉴定和16S rDNA测序，鉴定出IL-2菌为Pseudomonas属.考察了不同C/N质量浓度比、温度、溶解氧、接种量、处理时间对菌株IL-2反硝化脱氮效果的研究.随着C/N质量浓度比、处理时间、溶解氧的不断增加，菌株IL-2的脱氮效果逐渐增强；随着温度和接种量的不断增加，菌株IL-2的脱氮效果先逐渐增强后减弱.该菌株在初始硝态氮质量浓度为20 mg/L，C/N质量浓度比为10，温度为4 ℃，溶解氧为3.5 mg/L，接种量所占体积为30 %，处理10 h的条件下硝态氮的去除效果最好，为 95.95 % . 该菌株主要适用于冬季温度较低的市政污水的处理.
In this paper, a psychrotolerant aerobic denitrifying bacterium IL-2 was isolated from the activated sludge from the outlet of the CASS treatment process reaction tank of a sewage treatment plant at a temperature of 4 °C.Routine physiological and biochemical identification and 16S rDNA sequencing of strain IL-2 identified the strain as Pseudomonas.The effects of different mass concentration ratio of C/N, temperature, dissolved oxygen, inoculum amount and treatment time on denitrification of strain IL-2 were investigated.With the increase of mass concentration ratio of C/N, treatment time, and dissolved oxygen, the denitrification efficiency of IL-2 increased gradually.With the increase of temperature and inoculum amount, the denitrification effect of IL-2 first increased gradually and then ecreased.When the initial concentration of nitrate nitrogen was about 20 mg/L, the strain exhibited the best denitrification effect on the wastewater at a mass concentration ratio of C/N of 10, a temperature of 4 ℃, a dissolved oxygen of 3.5 mg/L.The volume of inoculation is 30 %, and a treatment time of 10 hours to achieve 95.95% degradation rate, indicating its main suitability for treatment of municipal sewage with low temperature in winter.
关键词
参考文献 10
- [1] HONG X, CHEN Z, ZHAO C, et al.Nitrogen Transformation Under Different Dissolved Oxygen Levels by the Anoxygenic Phototrophic Bacterium Marichromatium gracile [J]. World Journal of Microbiology and Biotechnology, 2017, 33(6):113-119. doi: 10.1007/s1 1/2-74-017-2280-z
- [2] CHEN S, HE S, WU C, et al.Characteristics of Heterotrophic Nitrification and Aerobic Denitrification Bacterium acznetobacter sp.T1 and Its Application for Pig Farm Wastewater Treatment [J]. Journal of Bioscience and Bioengineering, 2018, 127(2):201-205. doi: 10.1016/j.jbiosc.2018.07.025
- [3] FU G, YU T, HUANG L, et al.The Influence of Complex Fermentation Broth on Denitrification of Saline Sewage in Constructed Wetlands by Heterotrophic Nitrifying/aerobic Denitrifying Bacterial Communities [J]. Bioresource Technology, 2018, 250(2018):290-298. doi: 10.1016/j.biortech.2017.11.057
- [4] 樊琳, 产江为, 周波, 等.不同类型氮组成对异养硝化好氧反硝化体系中氮转化的影响 [J]. 山东农业大学学报(自然科学版), 2020, 51(5):833-839.doi：10.3969/j.issn.1000- 2324.2020.05.010 FAN Lin, CHAN Jiangwei, ZHOU Bo, et al.Effects of Different Types of Nitrogen Composition on Nitrogen Transformation in Heterotrophic Nitrification and Aerobic Denitrification System [J]. Journal of Shandong Agricultural University(Natural Science Edition), 2020, 51(5):833-839.
- [5] ROBERTSON L A, KUENEN J G.Aerobic Denitrification:A Controversy Revived [J]. Archives of Microbiology, 1984, 139(5):351-354. doi: 10.1007/bf00408378
- [6] 杨达, 赵婉宁, 陈越, 等.活性污泥法驯化一株好氧反硝化细菌 [J]. 首都师范大学学报(自然科学版), 2020, 41(6):31-35. doi: 10.19789/j.1004-9398.2020.06.006 YANG Da, ZHAO Wanning, CHEN Yue，et al.Domestication of an Aerobic Denitrifying Bacteria by Activated Sludge Method [J]. Journal of Capital Normal University (Natural Science Edition), 2020, 41(6):31-35.
- [7] 杨墨, 刘乾亮, 吕东伟, 等.低温异养硝化-好氧反硝化菌筛选及其脱氮特性 [J]. 中国给水排水, 2019, 35(23):100-104. doi: 10.19853/j.zgjsps.1000-4602.2019.23.019 YANG Mo, LIU Qianliang, LYU Dongwei，et al.Screening of Low-temperature Heterotrophic Nitrification-aerobic Denitrification Bacteria and Their Denitrification Characteristics [J]. China Water & Wastewater, 2019, 35(23):100-104.
- [8] ZHANG S M, SHA C Q, JIANG W，et al.Ammonium Removal at Low Temperature by a Newly Isolated Heterotrophic Nitrifying and Aerobic Denitrifying Bacterium Pseudomonas fluorescens Wsw-1001 [J]. Environmental Technology, 2015，36(19):2488-2494. doi: 10.1080/09593330.2015.1035759
- [9] 路俊玲，陈慧萍，肖琳.低温反硝化菌——施氏假单胞菌N3的筛选及脱氮性能 [J]. 环境科学，2018, 39(12):183-188. LU Junling, CHEN Huiping, XIAO Lin.Screening of Low-temperature Denitrifying Bacteria：Pseudomonas stutzeri N3 and Its Denitrification Performance [J].Environmental Science, 2018, 39(12):183-188.
- [10] HE T, YE Q, SUN Q, et al.Removal of Nitrate in Simulated Water at Low Temperature by a Novel Psychrotrophic and Aerobic Bacterium, Pseudomonas taiwanensis Strain [J]. BioMed Research International, 2018, 2018:1-9. doi: 10.1155/2018/4984087