期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
好氧耐盐反硝化菌的选育及脱氮性能
- (深圳市长隆科技有限公司 迈葳生物事业部，广州 深圳 518116)
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DOI：10.13763/j.cnki.jhebnu.nse.202204008
Breeding and Denitrification Performance of Aerobic Salt-tolerant Bacteria
摘要/Abstract
从活性污泥中分离出一株好氧耐盐反硝化菌YFX-6，耐盐度 10 %. 经生理生化鉴定和16S rDNA测序，鉴定出菌株YFX-6属于Halomonas sp. 考察了不同C/N质量浓度比、溶解氧、接种量、处理时间对菌株YFX-6在粮果实际废水中反硝化脱氮效果的研究. 随着C/N质量浓度比的不断增加，菌株YFX-6的反硝化脱氮效果先逐渐增强后又减弱；随着溶解氧、处理时间和接种量的不断增加，菌株YFX-6的脱氮效果逐渐增强后趋于稳定.初始硝态氮质量浓度约为 108.5 mg/L，氯化钠质量浓度为 10 mg/L，C/N质量浓度比为8，溶解氧为 3.5 mg/L，接种量所占体积分数为 20 %，处理 16 h 时，硝态氮去除率为 98.69 %. 因此，筛选出的一株好养耐盐的异氧反硝化菌可以在上述条件下表现出良好的脱氮性能.
A salt-tolerant bacterium YFX-6 was isolated from activated sludge with a salt tolerance of 10 %. After regular physiological and biochemical identification and 16S rDNA sequencing, the bacterium YFX-6 was identified to be Halomonas sp. The effects of different mass concentration ratio of C/N, dissolved oxygen, inoculum amount, and treatment time on the denitrification effect of strain YFX-6 in the treatment of the grain and fruit wastewater were investigated.With the increase of mass concentration ratio of C/N, the denitrification effect of strain YFX-6 first gradually increased and then decreased; with the grow of dissolved oxygen, treatment time and inoculum amount, the denitrification ability of strain YFX-6 gradually increased first and then keep constant.The initial nitrate nitrogen concentration, mass concentration ratio of C/N, dissolved oxygen and inoculation amount were 108.5 mg/L, 8, 3.5 mg/L and 20 %, respectively. After 16 h treatment, the nitrate removal rate was 98.69 %.Therefore, a strain of low-temperature heterotrophic denitrifying bacterium, which is tolerant to salinity and is good at nitrogen removal under appropriate conditions, was successfully screened.
关键词
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