河北师范大学学报—

利用AlamarBlue法对不同来源的真藓（Bryum argenteum Hedw.）甲醇提取物在体外分别对4种肿瘤细胞的增殖抑制作用进行评价.结果表明：不同来源的真藓甲醇提取物对于同种及不同种肿瘤细胞活性的影响不同.野外采集的真藓植株、改良Knop液体培养的真藓原丝体的甲醇提取物对人急性淋巴白血病细胞株Nalm 6和人胚肾细胞HEK293T的生长均表现抑制作用.2种来源的真藓甲醇提取物对HEK293T作用的EC₅₀值分别是15.12，2.14mg/mL，对Nalm 6作用的EC₅₀值分别是1.19，5.68mg/mL，对人食管鳞癌KYSE450细胞的增殖无抑制作用.

Abstract：

The AlamarBlue assay was used to evaluate the inhibitory effect of methanol extracts of Bryum argenteum Hedw.from different sources on the proliferation of four tumor cells in vitro.The results showed that the methanol extracts of B.argenteum from different sources had diverse effects on the proliferation of the same and different tumor cells.The growth of human acute lymphocytic leukemia cell line Nalm-6 and human embryonic kidney cell line HEK293T were inhibited by the methanol extracts from the gametophyte collected in the field and the protonema of B.argenteum cultured in the improved Knop liquid.The EC₅₀ values of the two kinds of B.argenteum methanol extracts on HEK293T were 15.12,2.14mg/mL,respectively,and the EC₅₀ on Nalm-6 were 1.19,5.68mg/mL,respectively.No inhibitory effect was observed on the proliferation of human esophageal squamous cell carcinoma KYSE450 cells.

关键词

关键词： 真藓 ; 增殖活性 ; 肿瘤细胞

Key words： Bryum argenteum Hedw. ; proliferative activity ; tumor cells

参考文献 14

[1] (明)李时珍.本草纲目(校点本) (第1册)[M].北京:人民卫生出版社,1977. LI Shizhen.Compendium of Materia Medica(Volume 1)[M].Beijing:People's Medical Publishing House,1977.
[2] (清)吴其濬.植物名实图考长编[M].上海:商务印书馆,1959. WU Qijun.Illustrated Catalogue of Plants[M].Shanghai:The Commercial Press,1959.
[3] 吴征镒.新华本草纲要(第3册)[M].上海:上海科学技术出版社,1990. WU Zhengyi.Xinhua Plantform for Materia Medica(Volume 3)[M].Shanghai:Shanghai Scientific & Technical Publishers,1990.
[4] 王沁凯.《新修本草》引用本草文献考略[J].中医文献杂志,2004,22(4):22-24.doi:10.3969/j.issn.1006-4737.2004.04.009 WANG Qinkai.A Brief Study on the Citation of Materia Medica in Newly Revised Materia Medica[J].Journal of Traditional Chinese Medicine Literature,2004,22(4):22-24.
[5] (宋)唐慎薇.证类本草[M].北京:中国医药科技出版社,2011. TANG Shenwei.Classified Materia Medica[M].Beijing:China Medical Science Press,2011.
[6] 韩国营,宋培浪,于美玲,等.中国药用苔藓植物研究的文献分析(Ⅱ)[J].贵州农业科学,2013,41(3):11-15.doi:10.3969/j.issn.1001-3601.2010.03.009 HAN Guoying,SONG Peilang,YU Meiling,et al.Analysis on Literatures of Chinese Medicinal Bryophytes (Ⅱ)[J].Guizhou Agricultural Sciences,2013,41(3):11-15.
[7] 于占鳌.银叶真藓和尖叶石灰藓治疗急性细菌性痢疾的初步观察[J].中级医刊,1959(5):14-15. YU Zhanao.Preliminary Observation on the Treatment of Acute Bacillary Dysentery by Bryum argenteum and Limulus acuminata[J].Chinese Journal of Medicine,1959(5):14-15.
[8] CHENG Xiaoxia,XIAO Yaping,WANG Xiaobing,et al.Anti-tumor and Pro-apoptotic Activity of Ethanolic Extract and Its Various Fractions from Polytrichum commune L ex Hedw in L1210 Cells[J].Journal of Ethnopharmacology,2012,143(1):49-56.doi:10.1016/j.jep.2012.05.054
[9] 成晓霞,张志琪,汤薇,等.大金发藓化学成分预试及其肿瘤细胞毒活性研究[J].天然产物研究与开发,2014,26(11):1733-1738.doi:10.16333/j.1001-6880.2014.11.027 CHENG Xiaoxia,ZHANG Zhiqi,TANG Wei,et al.Chemical Components and Cytotoxic Activity of Polytrichum commune L ex Hedw[J].Natural Products Research and Development,2014,26(11):1733-1738.
[10] 成晓霞,张志琪,肖娅萍,等.金发藓属化学成分及抗肿瘤作用研究进展[J].陕西师范大学学报(自然科学版),2014,42(5):55-59.doi:10.15983/j.cnki.jsnu.2014.05.035 CHENG Xiaoxia,ZHANG Zhiqi,XIAO Yaping,et al.Progress in the Studies on Chemical Constituents and Anti-tumor Effects of Polytrichum Spp[J].Journal of Shaanxi Normal University(Natural Science),2014,42(5):55-59.
[11] 郭玲,雷成康,袁文娟,等.大金发藓乙酸乙酯提取物对K562细胞膜的损伤效应[J].陕西师范大学学报(自然科学版),2016,44(5):89-93.doi:10.15983/j.cnki.jsnu.2016.05.055 GUO Ling,LEI Chengkang,YUAN Wenjuan,et al.Damage Effect of Ethyl Acetate Extract of Polytrichum commune on Membrane of Human Leukemia K562 Cell[J].Journal of Shaanxi Normal University(Natural Science),2016,44(5):89-93.
[12] ADAÍSES Simone Maciel da Silva,KÁTIA Cavalcanti Pôrto,ELIANA Akie Simabukuro.Effects of Light and Nutrients on Different Germination Phases of the Cosmopolitan Moss Bryum argenteum Hedw(Bryaceae)[J].Brazilian Archives of Biology and Technology,2010,53(4):763-769.doi:10.1590/S1516-89132010000400003
[13] 刘军珂,申珂琪,牛琳媛,等.银叶真藓外植体组织培养消毒方法的筛选[J].分子植物育种,2019,17(10):3326-3331.doi:10.13271/j.mpb.017.003326 LIU Junke,SHEN Keqi,NIU Linyuan,et al.Screening of Sterilizing Methods for Tissue Culture of Explants of Bryum argenteum[J].Molecluar Plant Breeding,2019,17(10):3326-3331.
[14] ZHANG Yong,WANG Danling,LICHTERVELDE de Lorenzo,et al.Functional Antibody CDR3 Fusion Proteins with Enhanced Pharmacological Properties[J].Angewandte Chemie,2013,52(32):8295-8298.doi:10.1002/ange.201303656