China is transforming potato into the country's fourth staple food,and the safety production of the crop directly relates to development of agricultural economy.During years of continually asexual propagation,potatoes can be infected by different virus that can reduce yield and tuber quality.Shoot tip culture is one of the most common and efficient method to obtain virus-free potato seedlings.In this study,we developed a simple and practicable strategy to regenerate virus-free seedlings by shoot tip tissue culture.It provide technology foundation for virus-free potato seedling fast propagation.