期刊信息
- 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
- 主办： 河北师范大学
- ISSN： 1000-5854
- CN： 13-1061/N
- 中国科技核心期刊
- 中国期刊方阵入选期刊
- 中国高校优秀科技期刊
- 华北优秀期刊
- 河北省优秀科技期刊
MDV中L-meq基因对MDCC-MSB1细胞端粒酶活性影响的研究
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1. 长春师范学院中心实验室, 吉林长春 130032;
2. 长春师范学院生命科学学院, 吉林长春 130032;
3. 吉林大学军需科技学院, 吉林长春 130062 -
DOI：
Study on the Effect of L-meq Gene of MDV on the Telomerase Activity in MDCC-MSB1 Cell
摘要/Abstract
构建了真核表达载体pcDNA3.1(+)-L-meq,利用脂质体2000转染MDCC-MSB1细胞,采用改进的端粒重复序列扩增程序(telomere repeat amplification protocol,TRAP)法、实时荧光定量RT-PCR方法检测转染前后端粒酶活性以及端粒酶反转录酶(telomerase reverse transcriptase,chTERT)chTERT mRNA的表达量的变化,探讨L-meq基因对细胞端粒酶活性的影响.结果表明L-meq基因在MDCC-MSB1细胞中能够稳定表达,L-MEQ的稳定表达能够下调chTERTmRNA的表达水平,并抑制了MDCC-MSB1细胞的端粒酶活性,使其相对端粒酶活力下降.
To characterize the properties and functions of L-meq gene,recombinant eukaryon expression vector pcDNA3.1(+)-L-meq was const ructed and transfected into MDCC-MSB1,a chicken lymp hoblastoid cell line transformed by Marek's disease virus by liposome 2000.Updated TRAP method and Real-time PCR was used to test the telomerase activity and ch TERT mRNA respectively before and after transfection.The results showed that,the cells transfected with pcDNA3.1(+)-L-meq stably expression L-M EQ protein even until 96 hours after transfection.The L-meq protein can suppress the expression of telomerase activity,softly down with the relative telomerase activity and also can depress the expression level of ch TERT.It is reasonable to presume that L-meq can be a transrepressor which suppress the telomerase activity by L-meq protein.