期刊信息

  • 刊名： 河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
  • 主办： 河北师范大学
  • ISSN： 1000-5854
  • CN： 13-1061/N
  • 中国科技核心期刊
  • 中国期刊方阵入选期刊
  • 中国高校优秀科技期刊
  • 华北优秀期刊
  • 河北省优秀科技期刊

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友情链接

一种过氧化氢无酶传感器的构建及性能研究

  • 1. 河北科技大学 理学院, 河北 石家庄 050018;
    2. 河北医科大学 基础医学部, 河北 石家庄 050017
  • DOI： 10.13763/j.cnki.jhebnu.nse.2016.05.009

Preparation and Properties of An Non-enzymatic Sensor for Hydrogen Peroxide

摘要/Abstract

摘要：

利用电化学方法将铂纳米粒子（PtNP）和普鲁士蓝（PB）固定到多壁碳纳米管（MWCNTs）修饰的玻碳电极（GCE）表面，成功构建了一种过氧化氢（H2O2）无酶电化学传感器.实验结果表明，在工作电位为-0.05V时，所制备的传感器对H2O2具有优良的电催化还原能力，响应时间小于5s，这主要归因于PB，PtNP和MWCNTs三者的协同作用.在5~4205μmol/L范围内H2O2的还原电流与其浓度呈线性关系，灵敏度为758μA/（mmol·L-1·cm2），检测限为0.30μmol/L（S/N=3）.此外，该传感器还具有优良的选择性、稳定性和重现性.

Abstract：

A novel non-enzymatic hydrogen peroxide electrochemical sensor is fabricated by immobilizing platinum nanoparticles(PtNP) and prussian blue(PB) onto the glassy carbon electrode(GCE) surface which has already modified with multi-walled carbon nanotubes (MWCNTs).The results demonstrated that the synergistic effect of PB,PtNP and MWCNTs can significantly improve the detection performance of the sensor for H2O2 at applied potential of -0.05V.The fabricated sensor provides linear amperometric responses for H2O2 in the concentration range of 5~4205μmol/L,with a sensitivity of 758 μA/(mmol·L-1·cm2) and a detection limit of 0.30 μmol/L(S/N=3).In addition,the sensor exhibits excellent selectivity,favorable stability and good reproducibility for H2O2 detection.

关键词

关键词： 多壁碳纳米管 ; 铂纳米粒子 ; 普鲁士蓝 ; 过氧化氢 ; 无酶传感器
Key words： multi-walled carbon nanotubes ; platinum nanoparticles ; prussian blue ; hydrogen peroxide ; non-enzymatic sensor

参考文献 30

  • [1] HUANG Y,LI S F Y.Electrocatalytic Performance of Silica Nanoparticles on Grapheme Oxide Sheets for Hydrogen Peroxide Sensing[J].Journal of Electroanalytical Chemistry,2013,690(1):8-12.doi:10.1016/j.jelechem.2012.11.041
  • [2] WEI H,WANG E K.Fe3O4 Magnetic Nanoparticles as Peroxidase Mimetics and Their Applications in H2O2 and Glucose Detection[J].Analytical Chemistry,2008,80(6):2250-2254.doi:10.1021/ac702203f.Epub 2008 Feb 22
  • [3] LI X H,ZHANG Z J,TAO L,et al.Sensitive and Selective Chemiluminescence Assay for Hydrogen Peroxide in Exhaled Breath Condensate Using Nanoparticle-based Catalysis[J].Spectrochimica Acta Part A,2013,107(7):311-316.doi:10.1016/j.saa.2013.01.062
  • [4] AIZAWA M,IKARIYAMA Y,KUNO K.Photovoltaic Determination of Hydrogen Peroxide with a Biophotodiode[J].Analytical Letters,1984,17(17):555-564.doi:10.1080/00032718408066489
  • [5] HURDIS E C,ROMEYN H Jr.Accuracy of Determination of Hydrogen Peroxide Bycerate Oxidimetry[J].Analytical Chemistry,1954,26(2):320-325.doi:10.1021/ac60086a016
  • [6] CATHCART R,SCHWIERS E,AMES B N.Detection of Picomole Levels of Hydroperoxides Using a Fluorescent Dichlorofluorescein Assay[J].Analytical Biochemistry,1983,134(1):111-116.doi:10.1016/0003-2697(83)90270-1
  • [7] TANG B,ZHANG L,XU K H.FIA-near-infrared Spectrofluorimetric Trace Determination of Hydrogen Peroxide Using Tricarchlorobocyanine Dye and Horseradish Peroxidase(HRP)[J].Talanta,2006,68(3):876-882.doi:10.1016/j.talanta.2005.06.053
  • [8] BO X J,BAI J,QI B,et al.Ultra-fine Pt Nanoparticles Supported on Ionic Liquid Polymer-functionalized Ordered Mesoporous Carbons for Nonenzymatic Hydrogen Peroxide Detection[J].Biosensoers and Bioelectronics,2011,28(1):77-83.doi:10.1016/j.bios.2011.07.001
  • [9] KUNG C C,LIN P Y,BUSE F J,et al.Preparation and Characterization of Three Dimensional Graphene Foam Supported Platinum-ruthenium Bimetallic Nanocatalysts for Hydrogen Peroxide Based Electrochemical Biosensors[J].Biosensoers and Bioelectronics,2014,52(2):1-7.doi:10.1016/j.bios.2013.08.025
  • [10] XU C X,WANG J P,ZHOU J H.Nanoporous PtNi Alloy As an Electrochemical Sensor for Ethanol and H2O2[J].Sensors and Actuators B,2013,182:408-415.doi:10.1016/j.snb.2013.03.035
  • [11] XIE L L,XU Y D,CAO X Y.Hydrogen Peroxide Biosensor Based on Hemoglobin Immobilized at Graphene,Flower-like Zinc Oxide,and Gold Nanoparticles Nanocomposite Modified Glassy Carbon Rlectrode[J].Colloids and Surfaces B,2013,107(107C):245-250.doi:10.1016/j.colsurfb.2013.02.020
  • [12] WANG L,ZHU H Z,SONG Y H,et al.Architecture of Poly(o-phenylenediamine)-Ag Nanoparticle Composites for a Hydrogen Peroxide Sensor[J].Electrochimica Acta,2012,60(1):314-320.doi:10.1016/j.electacta.2011.11.045
  • [13] 阮北,鲁彬,童汝亭,等.自组装疏基环肽单层膜修饰金电极电化学行为的研究[J].河北师范大学学报(自然科学版),2003,27(5):484-487.
  • [14] YOU J M,KIM D,JEON S.Electrocatalytic Reduction of H2O2 by Pt Nanoparticles Covalently Bonded to Thiolated Carbon Nanostructure[J].Electrochimica Acta,2012,65(1):288-293.doi:10.1016/j.electacta.2012.01.070
  • [15] GARJONYTE R,MALINAUSKAS A.Glucose Biosensor Based on Glucose Oxidase Immobilized in Electropolyerized Polyprrole and Poly(o-phenylenediammine) Films on a Prussian Blue-modified Electrode[J].Sensors and Actuators B,2000,63(1-2):122-128.doi:10.1016/S0925-4005(00)00317-8
  • [16] LI X,ZHU Q Y,TONG S F,et al.Self-assembled Microstructure of Carbon Nanotubes for Enzymeless Glucose Sensor[J].Sensors and Actuators B,2009,136(2):444-450.doi:10.1016/j.snb.2008.10.051
  • [17] HUANG K J,NIU D J,LIU X,et al.Direct Electrochemistry of Catalase at Amine-functionalized Graphene/gold Nanoparticles Composite Film for Hydrogen Peroxide Sensor[J].Electrochim Acta,2011,56(7):2947-2953.doi:10.1016/j.electacta.2010.12.094
  • [18] KARYAKIN A A,KARYAKINA E E,GORTON L.Amperometric Biosensor for Glutamate Using Prussian Blue-based "Artificial Peroxidase" As a Transducer for Hydrogen Peroxide[J].Analytical Chemistry,2000,72(2):1720-1723.doi:10.1021/ac990801o
  • [19] LIU X W,YAO Z J,WANG Y F,et al.Graphene Oxide Sheet-prussian Blue Nanocomposites:Green Synthesis and Their Extraordinary Electrochemical Properties[J].Colloids and Surfaces B,2010,81(2):508-512.doi:10.1016/j.colsurfb.2010.07.049
  • [20] ZHU X,NIU X H,ZHAO H L,et al.Doping Ionic Liquid into Prussian Blue-multiwalled Carbon Nanotubes Modified Screen-printed Electrode to Enhance the Nonenzymatic H2O2 Sensing Performance[J].Sensors and Actuators B,2014,195(5):274-280.doi:10.1016/j.snb.2014.01.052
  • [21] KAUSHIK A,KHAN R,SOLANKI P R,et al.Iron Oxide Nanoparticles-chitosan Composite Based Glucose Biosensor[J].Biosensors and Bioelectronics,2008,24(4):676-683.doi:10.1016/j.bios.2008.06.032
  • [22] ZHANG Y,SUN X M,ZHU L Z,et al.Electrochemical Sensing Based on Graphene Oxide/prussian Blue Hybrid Film Modified Electrode[J].Electrochimica Acta,2011,56(3):1239-1245.doi:10.1016/j.electacta.2010.11.011
  • [23] KEIHAN A H,SAJJADI S.Improvement of the Electrochemical and Electrocatalytic Behavior of Prussian Blue/carbon Nanotubes Composite via Ionic Liquid Treatment[J].Electrochimica Acta,2013,113(15):803-809.doi:10.1016/j.electacta.2013.07.063
  • [24] 李丽花,徐琴,王海燕,等.普鲁士蓝-多壁碳纳米管复合材料修饰电极测定过氧化氢[J].分析化学研究报告,2007,35(6):835-838.
  • [25] ZHU X,NIU X H,ZHAO H L,et al.Immobilization of Superoxide Dismutase on Pt-Pd/MWCNTs Hybrid Modified Electrode Surface for Superoxide Anion Detection[J].Biosensoers and Bioelectronics,2015,67:79-85.doi:10.1016/j.bios.2014.07.004
  • [26] BARD A J,FAULKNER L R.Electrochemical Methods:Fundamentals and Applications[M].2nd ed.New York:John Wiley and Sons,2001:580-631.
  • [27] 梁汝萍,谢慧英,邱建.基于普鲁士蓝电催化的三维多孔壳聚糖膜葡萄糖生物传感器的研制[J].分析试验室,2008,27(10):23-27.doi:10.13595/j.cnki.issn.1000-0720.2008.0286
  • [28] 张乐华,张华阳,李冲,等.普鲁士蓝/PDDA-石墨烯复合膜修饰电极的制备及应用于过氧化氢无酶传感器[J].传感技术学报,2014,27(4):438-445.doi:10.3969/j.issn.1004-1699.2014.04.004
  • [29] CHEN S H,MA L P,YUAN R,et al.Electrochemical Sensor Based on Prussian Blue Nanorods and Gold Nanochains for the Determination of H2O2[J].European Food Research and Technology,2011,232(1):87-95.doi:10.1007/s00217-010-1364-x
  • [30] ZHANG L,SONG Z N,ZHANG Q,et al.Enhancement of the Electrochemical Performance of Prussian Blue Modified Electrode via Ionic Liquid Treatment[J].Electroanalysis,2009,21(16):1835-1841.doi:10.1002/elan.200904620

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