河北师范大学学报—

期刊信息

刊名：河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
主办：河北师范大学
ISSN： 1000-5854
CN： 13-1061/N
中国科技核心期刊
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南天竹的离体培养研究

蔡鹏¹

苏华桃²

李丽炎²

刘华英²

姚惠文²

1. 广西师范大学漓江学院, 广西桂林 541004;
2. 广西师范大学生命科学学院, 广西桂林 541004
DOI： 10.13763/j.cnki.jhebnu.nse.2016.01.012

Study of in Vitro Culture of Nandina domestica Thunb

CAI Peng¹, SU Huatao², LI Liyan², LIU Huaying², YAO Huiwen²

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摘要/Abstract

摘要：

以南天竹茎段为材料,对不同消毒剂、消毒时间和内生菌抑制剂的消毒效果及诱导、增殖和生根培养基进行研究.结果表明:不同消毒剂消毒效果最佳时间为10min;消毒效果排序1g/L HgCl₂>50mL/L NaClO₂ >20mL/L NaClO₂ >80mL/L NaClO₂;适宜的诱导培养基为MS(murashige skoog培养基)+1mg/L 6 BA+0.1mg/L IBA+90mg/L青霉素+1600mg/L Vc,诱导率达66.67%;MS+0.5mg/L 6 BA+0.05mg/L NAA是最适增殖配方,株高1.67cm,增殖系数2.63;最佳壮苗培养基为MS+0.01mg/L NAA+0.3mg/L 6 BA,株高2.17cm,增殖系数6.27;在微量元素减半的1/2MS+0.2mg/L NAA+0.2mg/L IBA +4g/L活性碳+30g/L蔗糖的液体培养基上生根率可达76.67%.

Abstract：

Effects of different disinfectants,disinfection time and endophyte inhibitor,as well as various media for induction,proliferation and rooting were examined using stem segments of Nandina domestica as material. The results showed that 10min was the best for all disinfectants,with declining disinfection effect in the order of 1g/L HgCl₂,50mL/L NaClO₂,20mL/L NaClO₂ and 80mL/L NaClO₂.The optimum medium for induction was MS+1mg/L 6-BA+0.1mg/L IBA+90mg/L penicillin+1600mg/L Vc,with induction rate reaching 66.67%.The optimum multiplication formula was MS+0.5mg/L 6-BA+0.05mg/L NAA,on which the plant height averaged at 1.67cm and proliferation coefficient was as high as 2.63.The best shoot growth was observed on the medium of MS+0.01mg/L NAA+0.3mg/L 6-BA,with mean height of 2.17cm and proliferation coefficient of 6.27.Meanwhile,the highest rooting percentage of 76.67% was observed on the liquid medium of 1/2 MS (macroelements and microelements reduced by half)+0.2mg/L NAA+0.2mg/L IBA+4g/L activated charcoal+30g/L sucrose.

关键词

关键词： 南天竹 ; 离体培养 ; 无菌体系

Key words： Nandina domestica Thunb ; in vitro culture ; asepsis system

参考文献 16

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