河北师范大学学报—

期刊信息

刊名：河北师范大学学报（自然科学版）Journal of Hebei Normal University (Natural Science)
主办：河北师范大学
ISSN： 1000-5854
CN： 13-1061/N
中国科技核心期刊
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基于血红蛋白-石墨烯构建的过氧化氢传感器

翟牧牧¹

徐杰²

于伶俊²

崔荣静²

谷宁¹

1. 河北师范大学化学与材料科学学院, 河北石家庄 050024;
2. 常熟理工学院化学与材料工程学院, 江苏常熟 215500
DOI： 10.11826/j.issn.1000-5854.2014.06.013

Construction of Hydrogen Peroxide Sensor Based on Hemoglobin-graphene

ZHAI Mumu¹, XU Jie², YU Lingjun², CUI Rongjing², GU Ning¹

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摘要/Abstract

摘要：

用聚二甲基二烯丙基氯化铵(PDDA)将石墨烯(RGO)功能化,再与血红蛋白(Hb)超声混匀后滴涂到玻碳电极表面,用Nafion膜固定,得到Hb-PDDA-RGO/Nafion修饰的玻碳电极.研究了Hb的直接电化学,并构建了一种无媒介过氧化氢生物传感器.结果表明,该方法固定的Hb实现了直接电子传递,对过氧化氢的还原具有良好的电催化活性;构建的传感器对过氧化氢响应迅速,具有较宽的线性范围(0.1~350.2μmol/L)、较低的检出限(0.0364μmol/L)、较高的灵敏度(30.61μA/(mmol·L^-1·cm²))以及较低的米氏常数(18.1μmol/L).

Abstract：

Hemoglobin (Hb) was entrapped into graphene (RGO) that had been functioned with poly(diallyldimethylammonium chloride)(PDDA) under ultrasonic measurement.The direct electrochemistry of Hb was researched,and an unmediated biosensor for H₂O₂ reduction was developed by immobilizing Hb on a glassy carbon electrode modified with Hb-PDDA-RGO/Nafion.The results demonstrated that the immobilized Hb undergoes direct electron transfer and displays excellent bioelectrocatalytic activity for the reduction of H₂O₂.The sensor constructed by the Hb-PDDA-RGO/Nafion modified glassy carbon electrode displays an excellent response to the reduction of hydrogen peroxide with wider linear range from 0.1 to 350.2μmol/L,higher sensitivity with 30.61μA/(mmol·L^-1·cm²),lower detection limit with 0.0364μmol/L and lower Michaelis constant with 18.1μmol/L.

关键词

关键词： 石墨烯 ; 血红蛋白 ; 过氧化氢 ; 生物传感器 ; 直接电子传递

Key words： graphene ; hemoglobin ; hydrogen peroxide ; biosensor ; direct electron transfer

参考文献 15

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