在线阅读 --自然科学版 2003年4期《鸡传染性支气管炎病毒(IBV)H52疫苗株S1基因的克隆与序列测定》
鸡传染性支气管炎病毒(IBV)H52疫苗株S1基因的克隆与序列测定--[在线阅读]
朱毓永1,2, 张利平2, 赵宝华3
1. 承德民族师范专科学校化学系, 河北承德 067000;
2. 河北大学生命科学学院, 河北保定 071002;
3. 河北师范大学生命科学学院, 河北石家庄 050016
起止页码: 406--409页
DOI:
摘要
根据国外已发表的鸡传染性支气管炎病毒(IBV)S1基因序列,设计了一对引物并以RT-PCR特异性扩增出IBVH52疫苗株的S1基因,基因产物大小为1.62kb,与设计相符.对其进行序列测定后,与其他标准毒株H120,M41,BEAU株的S1基因进行同源性比较,结果表明,H52株与H120,M41和BEAU株的核苷酸序列的同源性分别为91.1%,96.9%和96.8%,由此可以看出,IBVH52疫苗株与标准毒株在S1基因上具有高度的同源性.

Sequencing and Homology Analysis of the S1 Gene of Vaccine Strain H52
ZHU Yu-yong1,2, ZHANG Li-ping2, ZHAO Bao-hua3
1. Department of Chemistry, Chengde Ethnol Normal School, Hebei Chengde 067000, China;
2. College of Life Science, Hebei University, Hebei Baoding 071002, China;
3. College of Life Science, Hebei Normal University, Hebei Shijiazhuang 050016, China
Abstract:
One pair of primers for amplifying the S1 gene of avian infectious bronchitis virus(IBV)had been designed,with which the predicted 1.62 kb fragment had been amplified via RT-PCR from IBV strain H52.The S1 gene of strain H52 was sequenced and compared with standard strains such as M41,H120 and BEAU.The results showed that the homologies of nucleic acids between H52 strain and M41,H120 and BEAU strains were 97.1%,96.9% and 96.8% respectively.The high homology of the S1 gene of IBV strain H52 with those of other IBV standard strains had been proved.

收稿日期: 2003-1-13
基金项目: 河北省教育厅博士基金资助项目(2002214)

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